Surface membrane CD4 turnover in phorbol ester stimulated T-lymphocytes. Evidence of degradation and increased synthesis

Autor:	Møller, B K, Andresen, B S, Christensen, E I, Petersen, C M
Jazyk:	angličtina
Rok vydání:	1990
Předmět:	Kinetics T-Lymphocytes Antibodies Monoclonal Humans Tetradecanoylphorbol Acetate Lymphocyte Activation Cells Cultured Antigens CD4
Zdroj:	Møller, B K, Andresen, B S, Christensen, E I & Petersen, C M 1990, ' Surface membrane CD4 turnover in phorbol ester stimulated T-lymphocytes. Evidence of degradation and increased synthesis ', F E B S Letters, vol. 276, no. 1-2, pp. 59-62 .
Popis:	Down-regulation of surface membrane CD4 (smCD4) in phorbol ester stimulated T-cells resulted from internalization. Internalization (T1/2 = 15 min at 50 ng PMA/ml) was followed by degradation of CD4-bound antibodies. Degradation in unstimulated T-cells was comparatively insignificant. Release of degradation products was PMA dose-dependent and could be inhibited by methylamine. Uptake and degradation continued after maximal down-regulation of surface membrane CD4, and methylamine did not inhibit reappearance of smCD4 antigens. Metabolic labelling of T-cells further showed that ongoing synthesis rather than recycling contributed to an accelerated smCD4 turnover in activated cells.
Databáze:	OpenAIRE
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