The cell-wide web coordinates cellular processes by directing site-specific Ca2+ flux across cytoplasmic nanocourses
| Autor: | Duan, Jingxian, Navarro-Dorado, Jorge, Clark, Jill H., Kinnear, Nicholas P., Meinke, Peter, Schirmer, Eric C., Evans, A. Mark |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cytosol/metabolism
Male Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism Nuclear Envelope Science Primary Cell Culture Cell Proliferation/physiology Calcium Signaling/physiology Cellular imaging Article Sarcoplasmic Reticulum Calcium-Transporting ATPases Rats Sprague-Dawley Ca2+ imaging Cytosol Sarcoplasmic Reticulum/metabolism Animals Muscle Cells/physiology Calcium Signaling lcsh:Science Muscle Skeletal Cells Cultured Nuclear Envelope/metabolism Cell Proliferation Muscle Cells Cell Membrane/metabolism Calcium signalling Cell Membrane Ryanodine Receptor Calcium Release Channel Rats Sarcoplasmic Reticulum Muscle Skeletal/cytology lcsh:Q Sprague-Dawley Ryanodine Receptor Calcium Release Channel/metabolism Muscle Contraction/physiology Muscle Contraction |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) Nature Communications Duan, J, Navarro-Dorado, J, Clark, J H, Kinnear, N P, Meinke, P, Schirmer, E C & Evans, A M 2019, ' The cell-wide web coordinates cellular processes by directing site-specific Ca2+ flux across cytoplasmic nanocourses ', vol. 10, no. 1, 2299 . https://doi.org/10.1038/s41467-019-10055-w |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-10055-w |
| Popis: | Ca2+ coordinates diverse cellular processes, yet how function-specific signals arise is enigmatic. We describe a cell-wide network of distinct cytoplasmic nanocourses with the nucleus at its centre, demarcated by sarcoplasmic reticulum (SR) junctions (≤400 nm across) that restrict Ca2+ diffusion and by nanocourse-specific Ca2+-pumps that facilitate signal segregation. Ryanodine receptor subtype 1 (RyR1) supports relaxation of arterial myocytes by unloading Ca2+ into peripheral nanocourses delimited by plasmalemma-SR junctions, fed by sarco/endoplasmic reticulum Ca2+ ATPase 2b (SERCA2b). Conversely, stimulus-specified increases in Ca2+ flux through RyR2/3 clusters selects for rapid propagation of Ca2+ signals throughout deeper extraperinuclear nanocourses and thus myocyte contraction. Nuclear envelope invaginations incorporating SERCA1 in their outer nuclear membranes demarcate further diverse networks of cytoplasmic nanocourses that receive Ca2+ signals through discrete RyR1 clusters, impacting gene expression through epigenetic marks segregated by their associated invaginations. Critically, this circuit is not hardwired and remodels for different outputs during cell proliferation. Although calcium signals are known to be critical for many cellular processes, how signaling elicits specific functions remains unclear. In visually striking work, Duan et al. reveal that networks of cytoplasmic nanocourses orchestrate cell activity by directing site-specific calcium signals. |
| Databáze: | OpenAIRE |
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