Drug Flux Across RPE Cell Models: The Hunt for An Appropriate Outer Blood–Retinal Barrier Model for Use in Early Drug Discovery

Autor: Hellinen, Laura, Hongisto, Heidi, Ramsay, Eva, Kaarniranta, Kai, Vellonen, Kati-Sisko, Skottman, Heli, Ruponen, Marika
Přispěvatelé: Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology, Tampere University, Drug Delivery Unit, Division of Pharmaceutical Biosciences, Drug Research Program
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Pharmaceutics
Pharmaceutics, Vol 12, Iss 2, p 176 (2020)
ISSN: 1999-4923
Popis: The retinal pigment epithelial (RPE) cell monolayer forms the outer blood−retinal barrier and has a crucial role in ocular pharmacokinetics. Although several RPE cell models are available, there have been no systematic comparisons of their barrier properties with respect to drug permeability. We compared the barrier properties of several RPE secondary cell lines (ARPE19, ARPE19mel, and LEPI) and both primary (hfRPE) and stem-cell derived RPE (hESC-RPE) cells by investigating the permeability of nine drugs (aztreonam, ciprofloxacin, dexamethasone, fluconazole, ganciclovir, ketorolac, methotrexate, voriconazole, and quinidine) across cell monolayers. ARPE19, ARPE19mel, and hfRPE cells displayed a narrow Papp value range, with relatively high permeation rates (5.2−26 × 10−6 cm/s. In contrast, hESC-RPE and LEPI cells efficiently restricted the drug flux, and displayed even lower Papp values than those reported for bovine RPE-choroid, with the range of 0.4−32 cm−6/s (hESC-RPE cells) and 0.4−29 × 10−6 cm/s, (LEPI cells). Therefore, ARPE19, ARPE19mel, and hfRPE cells failed to form a tight barrier, whereas hESC-RPE and LEPI cells restricted the drug flux to a similar extent as bovine RPE-choroid. Therefore, LEPI and hESC-RPE cells are valuable tools in ocular drug discovery.
Databáze: OpenAIRE
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