MTH1 Inhibitor TH1579 Induces Oxidative DNA Damage and Mitotic Arrest in Acute Myeloid Leukemia
| Autor: | Sanjiv, K., Calderón-Montaño, J.M., Pham, T.M., Erkers, T., Tsuber, V., Almlöf, I., Höglund, A., Heshmati, Y., Seashore-Ludlow, B., Nagesh Danda, A., Gad, H., Wiita, E., Göktürk, C., Rasti, A., Friedrich, S., Centio, A., Estruch, M., Våtsveen, T.K., Struyf, N., Visnes, T., Scobie, M., Koolmeister, T., Henriksson, M., Wallner, O., Sandvall, T., Lehmann, S., Theilgaard-Mönch, K., Garnett, M.J., Östling, P., Walfridsson, J., Helleday, T., Warpman Berglund, U. |
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| Rok vydání: | 2021 |
| Předmět: |
Mitosis
Apoptosis Mice SCID Mice Mice Inbred NOD hemic and lymphatic diseases Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured Animals Humans Cell Proliferation Cancer och onkologi Gene Expression Regulation Leukemic Cytarabine Prognosis Xenograft Model Antitumor Assays Phosphoric Monoester Hydrolases Leukemia Myeloid Acute DNA Repair Enzymes Pyrimidines Doxorubicin Cancer and Oncology Neoplastic Stem Cells Female Blast Crisis Reactive Oxygen Species |
| Zdroj: | Sanjiv, K, Calderón-Montaño, J M, Pham, T M, Erkers, T, Tsuber, V, Almlöf, I, Höglund, A, Heshmati, Y, Seashore-Ludlow, B, Nagesh Danda, A, Gad, H, Wiita, E, Göktürk, C, Rasti, A, Friedrich, S, Centio, A, Estruch, M, Våtsveen, T K, Struyf, N, Visnes, T, Scobie, M, Koolmeister, T, Henriksson, M, Wallner, O, Sandvall, T, Lehmann, S, Theilgaard-Mönch, K, Garnett, M J, Östling, P, Walfridsson, J, Helleday, T & Warpman Berglund, U 2021, ' MTH1 Inhibitor TH1579 Induces Oxidative DNA Damage and Mitotic Arrest in Acute Myeloid Leukemia ', Cancer Research, vol. 81, no. 22, pp. 5733-5744 . https://doi.org/10.1158/0008-5472.CAN-21-0061 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | Acute myeloid leukemia (AML) is an aggressive hematologic malignancy, exhibiting high levels of reactive oxygen species (ROS). ROS levels have been suggested to drive leukemogenesis and is thus a potential novel target for treating AML. MTH1 prevents incorporation of oxidized nucleotides into the DNA to maintain genome integrity and is upregulated in many cancers. Here we demonstrate that hematologic cancers are highly sensitive to MTH1 inhibitor TH1579 (karonudib). A functional precision medicine ex vivo screen in primary AML bone marrow samples demonstrated a broad response profile of TH1579, independent of the genomic alteration of AML, resembling the response profile of the standard-of-care treatments cytarabine and doxorubicin. Furthermore, TH1579 killed primary human AML blast cells (CD45+) as well as chemotherapy resistance leukemic stem cells (CD45+Lin−CD34+CD38−), which are often responsible for AML progression. TH1579 killed AML cells by causing mitotic arrest, elevating intracellular ROS levels, and enhancing oxidative DNA damage. TH1579 showed a significant therapeutic window, was well tolerated in animals, and could be combined with standard-of-care treatments to further improve efficacy. TH1579 significantly improved survival in two different AML disease models in vivo. In conclusion, the preclinical data presented here support that TH1579 is a promising novel anticancer agent for AML, providing a rationale to investigate the clinical usefulness of TH1579 in AML in an ongoing clinical phase I trial. Significance: The MTH1 inhibitor TH1579 is a potential novel AML treatment, targeting both blasts and the pivotal leukemic stem cells while sparing normal bone marrow cells. |
| Databáze: | OpenAIRE |
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