Micronuclei Assessment of The Radioprotective Effects of Melatonin and Vitamin C in Human Lymphocytes
| Autor: | Rostami, Aram, Moosavi, Seyed Akbar, Dianat Moghadam, Hassan, Bolookat, Eftekhar Rajab |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 18, Iss 1, Pp 46-51 (2016) Cell Journal (Yakhteh) |
| ISSN: | 2228-5814 2228-5806 |
| Popis: | Objective: Critical macromolecules such as DNA maybe damaged by free radicals that are generated from the interaction of ionizing radiation with biological systems. Melatonin and vitamin C have been shown to be direct free radical scavengers. The aim of this study was to investigate the in vivo/in vitro radioprotective effects of melatonin and vitamin C separately and combined against genotoxicity induced by 6 MV x-ray irradiation in human cultured blood lymphocytes. Materials and Methods: In this experimental study, fifteen volunteers were divided into three groups of melatonin, vitamin C and melatonin plus vitamin C treatment. Peripheral blood samples were collected from each group before, and 1, 2 and 3 hours after melatonin and vitamin C administration (separately and combined). The blood samples were then irradiated with 200 cGy of 6 MV x-ray. In order to characterize chromosomal aberrations, the lymphocyte samples were cultured with mitogenic stimulus on cytokinesisblocked binucleated cells. Results: The samples collected 1hour after melatonin and vitamin C (separately and combined) ingestion exhibited a significant decrease in the incidence of micronuclei compared with their control group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|