CD40-mediated stimulation contributes to lymphocyte proliferation, antibody production, eosinophilia, and mastocytosis during an in vivo type 2 response, but is not required for T cell IL-4 production
| Autor: | Lu P, Joseph Urban, Xd, Zhou, Sj, Chen, Madden K, Moorman M, Nguyen H, Sc, Morris, Fd, Finkelman, Wc, Gause |
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| Předmět: |
B-Lymphocytes
Mice Inbred BALB C Nematospiroides dubius Membrane Glycoproteins T-Lymphocytes CD40 Ligand Antibodies Helminth Antibodies Monoclonal Lymphocyte Activation Binding Competitive Mice Gene Expression Regulation Immunoglobulin G Eosinophilia Hypersensitivity Animals Cytokines Female Interleukin-4 CD40 Antigens Mastocytosis Strongylida Infections |
| Zdroj: | Europe PubMed Central |
| Popis: | CD40/CD40 ligand interactions are required for the development of T cell-dependent Ab responses in vivo. The role of these cell surface molecules in contributing to T cell cytokine production and the development of effector populations other than B cells and T cells is, however, less well defined. We have examined the in vivo effects of blocking CD40/CD40 ligand interactions on the type 2 mucosal immune response that follows oral inoculation of mice with the nematode parasite, Heligmosomoides polygyrus. Administration of anti-gp39 (CD40L) mAb (MR1) blocked H. polygyrus-induced elevations in serum IgG1 levels and inhibited elevations in blood eosinophils and mucosal mast cells at day 14 after inoculation. Anti-gp39 mAb markedly inhibited B cell blastogenesis 8 days after H. polygyrus inoculation but did not inhibit elevations in B cell class II MHC expression. Maximal elevations in B7-2 expression required signaling through both CD40 and the IL-4R. Elevations in T cell cytokine gene expression and elevations in the number of IL-4-secreting cells were unaffected by treatment with anti-gp39 mAb, although IL-4 production was inhibited by anti-IL-4R mAb. These results suggest that CD40/CD40L interactions are not required to activate T cells to produce cytokines but are required for the activation and proliferation of other effector cells associated with the type 2 response. |
| Databáze: | OpenAIRE |
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