Histone Modifications of H3K4me3, H3K9me3 and Lineage Gene Expressions in Chimeric Mouse Embryo
| Autor: | Maryam Salimi, Abolfazl Shirazi, Mohsen Norouzian, Mohammad Mehdi Mehrazar, Mohammad Mehdi Naderi, Mohammad Ali Shokrgozar, Mirdavood Omrani, Seyed Mahmoud Hashemi |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Cell Journal (Yakhteh) Cell Journal, Vol 22, Iss 1, Pp 96-105 (2020) |
| ISSN: | 2228-5806 |
| Popis: | Objective Chimeric animal exhibits less viability and more fetal and placental abnormalities than normal animal. This study was aimed to determine the impact of mouse embryonic stem cells (mESCs) injection into the mouse embryos on H3K9me3 and H3K4me3 and cell lineage gene expressions in chimeric blastocysts. Materials And Methods In our experiment, at the first step, incorporation of the GFP positive mESCs (GFP-mESCs) 129/Sv into the inner cell mass (ICM) of pre-compacted and compacted morula stage embryos was compared. At the second and third steps, H3K4me3 and H3K9me3 status as well as the expression of Oct4, Nanog, Tead4, and Cdx2 genes were determined in the following groups: i. In vitro blastocyst derived from in vivo morula subjected to mESCs injection (blast/chimeric), ii. In vivo derived blastocyst (blast/in vivo), iii. In vitro blastocyst derived from culture of morula in vivo (blast/morula), and iv. In vitro blastocyst derived from morula in vivo subjected to sham injection (blast/sham). Results Subzonal injection of GFP-mESCs at the pre-compacted embryos produced more chimeric blastocysts than compacted embryos (P |
| Databáze: | OpenAIRE |
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