Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles
| Autor: | Lizette Morejón, José Angel Delgado, Alexandre Antunes Ribeiro, Marize Varella de Oliveira, Eduardo Mendizábal, Ibrahim García, Adrián Alfonso, Patrina Poh, Martijn van Griensven, Elizabeth R. Balmayor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Calcium Phosphates
GROWTH-FACTOR BIOCERAMICS Cell Survival Biocompatible Materials Article VIVO lcsh:Chemistry Tissue Scaffolds/chemistry DELIVERY DESIGN Materials Testing Cell Adhesion Humans biphasic calcium phosphates BIOMATERIALS bone tissue engineering lcsh:QH301-705.5 Cells Cultured Biocompatible Materials/chemistry Tissue Scaffolds Durapatite/chemistry Tissue Engineering Mesenchymal Stem Cells/cytology POROSITY hydroxyapatite Mesenchymal Stem Cells MECHANICAL-PROPERTIES β-tricalcium phosphate Durapatite lcsh:Biology (General) lcsh:QD1-999 TISSUE scaffolds Nanoparticles Calcium Phosphates/chemistry Nanoparticles/chemistry beta-tricalcium phosphate |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 7 International journal of molecular sciences, 20(7):1790. Multidisciplinary Digital Publishing Institute (MDPI) International Journal of Molecular Sciences, Vol 20, Iss 7, p 1790 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20071790 |
| Popis: | Ceramic materials mimic the mineral composition of native bone and feature osteoconductive properties they are therefore used to regenerate bone tissue. Much research focuses on increasing the porosity and pore interconnectivity of ceramic scaffolds to increase osteoconductivity, cell migration and cell-cell interaction. We aimed to fabricate biocompatible 3D-scaffolds featuring macro- and microporous calcium phosphates with high pore interconnection. Nanoparticles of hydroxyapatite (HA) and calcium deficient hydroxyapatite (CDHA) were synthesized by wet chemical precipitation. Scaffolds were produced from them by the replication polymeric foam technique. Solid content and sintering temperature were varied. Nanoparticles and scaffolds were characterized regarding morphology, chemical and mineral composition, porosity and mechanical properties. Biocompatibility, cell attachment and distribution were evaluated in vitro with human adipose mesenchymal stem cells. Scaffolds with total porosity of 71%&ndash 87%, pores in the range of 280&ndash 550 µ m and connectivity density up to 43 mm&minus 3 were obtained. Smaller pore sizes were obtained at higher sintering temperature. High solid content resulted in a decrease of total porosity but increased interconnectivity. Scaffolds 50HA/50&beta TCP featured superior interconnectivity and mechanical properties. They were bioactive and biocompatible. High HA solid content (40 wt.%) in the HA pure scaffolds was negative for cell viability and proliferation, while in the 50HA/50&beta TCP composite scaffolds it resulted more biocompatible. |
| Databáze: | OpenAIRE |
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