Cloning and characterization of a novel activating Ly49 closely related to Ly49A
| Autor: | Ap, Makrigiannis, Gosselin P, Lh, Mason, Ls, Taylor, Daniel McVicar, Jr, Ortaldo, Sk, Anderson |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Complementary Base Sequence Molecular Sequence Data Antibodies Monoclonal Membrane Proteins Mice Inbred Strains Lymphocyte Activation Polymerase Chain Reaction Antigen-Antibody Reactions Killer Cells Natural Mice Animals Antigens Ly Humans Lectins C-Type Amino Acid Sequence Calcium Signaling Cloning Molecular Phosphorylation Receptors Immunologic Carrier Proteins NK Cell Lectin-Like Receptor Subfamily A Adaptor Proteins Signal Transducing Receptors NK Cell Lectin-Like |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-1767 |
| Popis: | The majority of the known Ly49 family members have been isolated from either C57BL/6 (B6) or BALB/c mice. Interestingly, the anti-Ly49 Ab reactivities observed in 129/J mice are different from those of B6 mice. Furthermore, immunoprecipitation of 129/J NK cell lysates with YE1/32 and YE1/48, Abs specific for the inhibitory Ly49A in B6, resulted in detection of the activation-associated DAP12 molecule. These results indicated a need for a more detailed study of this strain. Therefore, a cloning strategy was devised to isolate Ly49 cDNAs from 129/J mice. An immunoreceptor tyrosine-based inhibitory motif-containing, Ly49D-related clone was discovered that we have named Ly49O, and one immunoreceptor tyrosine-based inhibitory motif-lacking, Ly49A-related clone was discovered that we have named Ly49P. No anti-Ly49 mAb reacted with Ly49O, whereas the molecule encoded by the Ly49P cDNA was found to react with YE1/32 and YE1/48. Ly49P was found to associate with mouse DAP12, and Ab-mediated cross-linking of Ly49P resulted in mouse DAP12 phosphorylation and Ca2+ mobilization, indicating that Ly49P is a competent activation receptor. Ly49P, therefore, represents a novel member of the Ly49 activating receptor subfamily. |
| Databáze: | OpenAIRE |
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