Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments

Autor:	Di Leo, S., Marni, S., Plata, C.A., Fraccia, T.P., Smith, G.P., Maritan, A., Suweis, S., Bellini, T.
Rok vydání:	2022
Předmět:	Solutions Nucleic Acid Conformation Nucleic Acid Hybridization Temperature Thermodynamics DNA Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)
Zdroj:	PLoS computational biology. 18(4)
ISSN:	1553-7358
Popis:	Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. Here we describe, both experimentally and theoretically, the formation of duplexes in a solution of random-sequence DNA (rsDNA) oligomers of length L = 8, 12, 20 nucleotides. rsDNA solutions are formed by 4L distinct molecular species, leading to a variety of pairing motifs that depend on sequence complementarity and range from strongly bound, fully paired defectless helices to weakly interacting mismatched duplexes. Experiments and theory coherently combine revealing a hybridization statistics characterized by a prevalence of partially defected duplexes, with a distribution of type and number of pairing errors that depends on temperature. We find that despite the enormous multitude of inter-strand interactions, defectless duplexes are formed, involving a fraction up to 15% of the rsDNA chains at the lowest temperatures. Experiments and theory are limited here to equilibrium conditions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f7fda82ab5eb91fddb8c06984dd40d83 https://pubmed.ncbi.nlm.nih.gov/35404933 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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