Staphylococcal enterotoxin-dependent lysis of MHC class II negative target cells by cytolytic T lymphocytes
| Autor: | Herrmann T, Romero P, Silvia Sartoris, Paiola F, Rs, Accolla, Jl, Maryanski, Hr, Macdonald |
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| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Mice Inbred BALB C Staphylococcus aureus Lymphoma B-Cell Dose-Response Relationship Immunologic Histocompatibility Antigens Class II Mast-Cell Sarcoma In Vitro Techniques Blotting Northern Flow Cytometry Lymphocyte Activation Cell Line Enterotoxins Epitopes Mice Animals Humans T-Lymphocytes Cytotoxic |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-1767 |
| Popis: | The enterotoxins of Staphylococcus aureus (SE) are extremely potent activators of human and mouse T lymphocytes. In general, T cell responses to SE are MHC class II dependent (presumably reflecting the ability of SE to bind directly to MHC class II molecules) and restricted to responding cells expressing certain T cell receptor beta-chain variable (TCR V beta) domains. Recently we demonstrated that CD8+ CTL expressing appropriate TCR V beta could recognize SE presented on MHC class II-bearing target cells. We now show that MHC class II expression is not strictly required for T cell recognition of SE. Both human and mouse MHC class II negative target cells could be recognized (i.e., lysed) in a SE-dependent fashion by CD8+ mouse CTL clones and polyclonal populations, provided that the CTL expressed appropriate TCR V beta elements. SE-dependent lysis of MHC class II negative targets by CTL was inhibited by mAb directed against CD3 or LFA-1, suggesting that SE recognition was TCR and cell contact dependent. Furthermore, different SE were recognized preferentially by CTL on MHC class II+ vs MHC class II- targets. Taken together, our data raise the possibility that SE binding structures distinct from MHC class II molecules may exist. |
| Databáze: | OpenAIRE |
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