Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest
| Autor: | Blottner, A., Bosutti, A., Degens, H., Schiffl, G., Gutsmann, M., Buehlmeier, J., Rittweger, J., Bergita Ganse, Heer, M., Salanova, M. |
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| Přispěvatelé: | Dieter, Blottner, Bosutti, Alessandra, Hans, Degen, Gudrun, Schiffl, Martina, Gutsmann, Judith, Buehlmeier, Joern, Rittweger, Bergita, Ganse, Martina, Heer, Michele, Salanova |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Muscle Atrophy Cross-Over Studies Potassium Compounds Proteolysis Biomarkers Bed Rest Whey Protein Disuse Muscle Atrophy Proteolysis Biomarkers Milk Proteins Immunohistochemistry Whey Protein Bicarbonates Muscular Atrophy Young Adult Whey Proteins Dietary Supplements Proteolysis Humans Disuse Bed Rest Weltraumphysiologie |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| Popis: | Objectives: To investigate the effect of whey protein plus potassium bicarbonate supplement on disused skeletal muscle structure and proteolysis after bed rest (BR). Methods: Soleus (SOL) and vastus lateralis (VL) biopsies were sampled from ten (n=10) healthy male subjects (aged 31±6 years) who did BR once with and once without protein supplement as a dietary countermeasure (cross-over study design). The structural changes (myofibre size and type distribution) were analysed by histological sections, and muscle protein breakdown indirectly via the proteolysis markers, calpain 1 and 3, calpastatin, MuRF1 and 2, both in muscle homogenates and by immunohistochemistry. Results: BR caused size-changes in myofiber cross-sectional area (FCSA, SOL, p=0,004; VL, p=0.03), and myofiber slow-to-fast type transition with increased hybrids (SOL, p=0.043; VL, p=0.037) however with campaign differences in SOL (p |
| Databáze: | OpenAIRE |
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