Hyaluronic acid nanohydrogels as a useful tool for BSAO immobilization in the treatment of melanoma cancer cells
| Autor: | Montanari, Elita, Capece, Sabrina, Di Meo, Chiara, Meringolo, Martina, Coviello, Tommasina, Agostinelli, Enzo, Matricardi, Pietro |
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| Přispěvatelé: | Department of Drug Chemistry and Technologies, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Biochemical Sciences 'Rossi Fanelli', Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], This work was financially supported by Sapienza University of Rome ‘‘Ricerche Universitarie’’ 2011, no. C26A119N2S (P.M.), by the Italian MIUR(Ministero dell’Istruzione, dell’Università e della Ricerca, E.A.), by Istituto Superiore di Sanità a ‘‘Project Italy-USA’’ (E.A.), and byIstituto Pasteur-Fondazione Cenci Bolognetti (E.A.). |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Oxidation-Reduction
MESH: Cell Death MESH: Cell Line Tumor MESH: Enzymes Immobilized MESH: Melanoma [SDV]Life Sciences [q-bio] Static Electricity Nanogels [SDV.CAN]Life Sciences [q-bio]/Cancer MESH: Polyethyleneimine Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols MESH: Cholesterol Cell Line Tumor Animals Humans Polyethyleneimine MESH: Animals MESH: Particle Size Hyaluronic Acid Particle Size Melanoma MESH: Static Electricity MESH: Hydrogel MESH: Humans MESH: Hyaluronic Acid Cell Death Enzymes Immobilized MESH: Cattle Cholesterol MESH: Polyethylene Glycols MESH: Amine Oxidase (Copper-Containing) Cattle Amine Oxidase (Copper-Containing) Oxidation-Reduction |
| Zdroj: | Macromolecular Bioscience Macromolecular Bioscience, Wiley-VCH Verlag, 2013, 13 (9), pp.1185-94. ⟨10.1002/mabi.201300114⟩ |
| ISSN: | 1616-5187 1616-5195 |
| DOI: | 10.1002/mabi.201300114⟩ |
| Popis: | International audience; An alternative anticancer therapy based on the use of bovine serum amine oxidase (BSAO), an enzyme that converts polyamines over-expressed in malignant cells, into hydrogen peroxide and aldehyde(s), thus inducing high cytotoxicity in cancer cells, was recently proposed. With the aim of improving the system efficacy by exploiting a nanotechnology approach, BSAO is covalently immobilized onto injectable nanohydrogels (NHs) based on cholesterol-graft-hyaluronic acid (HA-CH), a biocompatible conjugate that spontaneously leads to self-assembled structures in aqueous solutions. In this study, the physicochemical properties of the HA-CH-based NHs and the NHs cytocompatibility are reported. The properties of the NHs-BSAO system are also studied in terms of protein residual activity, both in vitro and on a model melanoma cell line. |
| Databáze: | OpenAIRE |
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