Disruption of putative anion channel gene AtCLC-a in Arabidopsis suggests a role in the regulation of nitrate content

Autor: Geelen, D., Claire Lurin, David Bouchez, Frachisse, J. M., François Lelièvre, Béatrice Courtial, Barbier-Brygoo, H., Maurel, C.
Přispěvatelé: ProdInra, Migration, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2000
Předmět:
DNA
Bacterial
MESH: Sequence Homology
Amino Acid
Molecular Sequence Data
Arabidopsis
MESH: Sequence Alignment
MESH: Amino Acid Sequence
MESH: Arabidopsis Proteins
MESH: Base Sequence
ADN-T
Genes
Plant
MESH: Sequence Homology
Nucleic Acid
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
MESH: Recombinant Proteins
Chloride Channels
Sequence Homology
Nucleic Acid
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
MESH: Gene Library
MESH: Genes
Plant
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
MESH: Arabidopsis
Amino Acid Sequence
ComputingMilieux_MISCELLANEOUS
Gene Library
Plant Proteins
Nitrates
MESH: Molecular Sequence Data
Base Sequence
Sequence Homology
Amino Acid
MESH: Plant Proteins
Arabidopsis Proteins
MESH: Chloride Channels
MESH: DNA
Bacterial
Recombinant Proteins
Mutagenesis
Insertional
MESH: Mutagenesis
Insertional
MESH: Nitrates
Agrobacterium tumefaciens
MESH: Rhizobium radiobacter
Sequence Alignment
Zdroj: Plant Journal
Plant Journal, Wiley, 2000, 21 (3), pp.259-67. ⟨10.1046/j.1365-313x.2000.00680.x⟩
HAL
Plant Journal, Wiley, 2000, 21 (3), pp.259-267
ISSN: 0960-7412
1365-313X
DOI: 10.1046/j.1365-313x.2000.00680.x⟩
Popis: In animals and yeast, voltage-dependent chloride channels of the CLC family play a role in basic cellular functions such as epithelial transport, plasma membrane excitability, and control of pH and membrane potential in intracellular compartments. To assess the function of CLCs in plants, we searched for CLC insertion mutants in a library of Arabidopsis lines transformed by Agrobacterium tumefaciens transferred DNA (T-DNA). Using a polymerase chain reaction-based screening procedure, an Arabidopsis line that carries a T-DNA insertion within the C-terminus of the AtCLC-a coding sequence was identified. Progeny from this plant line, clca-1, showed dramatically altered transcription of the AtCLC-a gene. Plants homozygous for the clca-1 mutation exhibited normal development and a morphology indistinguishable from the wild-type. However, their capacity to accumulate nitrate under conditions of nitrate excess was reduced in roots and shoots, by approximately 50%, while chloride, sulphate and phosphate levels were similar to the wild-type. In addition, the herbicide chlorate, an analogue of nitrate, induced a faster and more pronounced chlorosis in mutant plants. Hypersensitivity to chlorate as well as decreased nitrate levels co-segregated with the T-DNA insertion. They were found at various time points of the clca-1 life cycle, supporting the idea that AtCLC-a has a general role in the control of the nitrate status in Arabidopsis. Concordant with such a function, AtCLC-a mRNA was found in roots and shoots, and its levels rapidly increased in both tissues upon addition of nitrate but not ammonium to the culture medium. The specificity of AtCLC-a function with respect to nitrate is further supported by a similar free amino acid content in wild-type and clca-1 plants. Although the cellular localization of AtCLC-a remains unclear, our results suggest that AtCLC-a plays a role in controlling the intracellular nitrate status.
Databáze: OpenAIRE
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