Acute molecular effects of pressure-controlled intermittent coronary sinus occlusion in patients with advanced heart failure

Autor: Mohl, Werner, Spitzer, Ernest, Mader, Robert M., Wagh, Vilas, Nguemo, Filomain, Milasinovic, Dejan, Jusić, Alem, Khazen, Cesar, Szodorai, Edit, Birkenberg, Beatrice, Lubec, Gert, Hescheler, Juergen, Serruys, Patrick W.
Přispěvatelé: Cardiology
Rok vydání: 2018
Předmět:
Zdroj: ESC heart failure, 5(6), 1176-1183. The Heart Failure Association of the European Society of Cardiology
ESC Heart Failure
ISSN: 2055-5822
Popis: Aims Cardiac repair has steered clinical attention and remains an unmet need, because available regenerative therapies lack robust mechanistic evidence. Pressure‐controlled intermittent coronary sinus occlusion (PICSO), known to induce angiogenetic and vasoactive molecules as well as to reduce regional ischemia, may activate endogenous regenerative processes in failing myocardium. We aimed to investigate the effects of PICSO in patients with advanced heart failure undergoing cardiac resynchronization therapy. Methods and results Eight out of 32 patients were treated with PICSO, and the remainder served as controls. After electrode testing including left ventricular leads, PICSO was performed for 20 min. To test immediate molecular responses, in both patient groups, coronary venous blood samples were taken at baseline and after 20 min, the time required for the intervention. Sera were tested for microRNAs and growth factors. To test the ability of up‐regulated soluble factors on cell proliferation and expression of transcription factors [e.g. Krüppel‐like factor 4 (KLF‐4)], sera were co‐cultured with human cardiomyocytes and fibroblasts. As compared with controls, significant differential expression (differences between pre‐values and post‐values in relation to both patient cohorts) of microRNA patterns associated with cardiac development was observed with PICSO. Importantly, miR‐143 (P
Databáze: OpenAIRE
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