Tea catechins induce crosstalk between signaling pathways and stabilize mast cells in ulcerative colitis
| Autor: | A, Gerges Geagea, M, Rizzo, A, Eid, I, Hajj Hussein, Z, Zgheib, M N, Zeenny, R, Jurjus, M L, Uzzo, G F, Spatola, G, Bonaventura, A, Leone, L, Massaad-Massade, A, Jurjus |
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| Přispěvatelé: | Gerges Geagea, A., Rizzo, M., Eid, A., Hajj Hussein, I., Zgheib, Z., Zeenny, M.N., Jurjus, R., Uzzo, M.L., Spatola, G.F., Bonaventura, G., Leone, A., Massaad-Massade, L., Jurjus, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Cancer Research Colon Physiology Endocrinology Diabetes and Metabolism Immunology Anti-Inflammatory Agents Antioxidants Catechin Rats Sprague-Dawley Endocrinology Physiology (medical) Animals Immunology and Allergy Mast Cells Tea Interleukin-6 Tumor Necrosis Factor-alpha NF-kappa B Colitis Rats Gene Expression Regulation Trinitrobenzenesulfonic Acid Oncology Reactive Oxygen Species Signal Transduction |
| Popis: | It is well documented that nutraceuticals, in general, and Green tea catechins, in particular, possess a potential therapeutic value in inflammatory bowel diseases (IBD) due to their anti-oxidative and anti-inflammatory effects. This study aimed to investigate the possible mechanism of action of catechins in a rat model of colitis induced by 2.4.6 trinitrobenzene sulfonic acid (TNBS). Thirty-five young adult Sprague-Dawley rats were divided into four groups: normal control (n=5), catechins (n=9), TNBS (n=9) and TNBS plus catechins (n=12) treated. Catechin in the form of Epigallocatechin-3-gallate (EGCG) was administered daily by intraperitoneal injection, 1 week before the induction date of UC. Biopsies of the descending colon were collected on days 3, 10 and 17, and partly frozen for molecular studies or fixed for light microscopy. The status of intestinal tissue alterations and mast cells number were also assessed, as well as the mRNA expressions of IL-6, TNF-a and NF-kB, and determination of ROS expression. Histological data depicted a significant amelioration in the TNBS- and EGCG-treated rats compared to the non-treated animals. Catechin expressed strong anti-inflammatory and anti-oxidant effects, ameliorated ulcerative colitis and stabilized mast cells. The mechanism of action occurred basically through the NF-kB pathway and possibly through a crosstalk with other pathways. |
| Databáze: | OpenAIRE |
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