In Vitro Macrophage Nitric Oxide and Interleukin-1 Beta Suppression by Moringa peregrina Seed
| Autor: | Shaymaa Fadhel Abbas ALBAAYIT, Ahmed Salim Kadhim AL-KHAFAJI, Hiba Sarmed ALNAIMY |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Turkish Journal of Pharmaceutical Sciences Turkish Journal of Pharmaceutical Sciences, Vol 16, Iss 3, Pp 362-365 (2019) |
| ISSN: | 2148-6247 1304-530X |
| Popis: | Objectives: Moringa peregrina has long been used in folk medicine to treat diseases including fever, headache, burns, constipation, gut pains, and inflammation. Nitric oxide (NO) and interleukin-1β (IL-1β) play an important role in the pathophysiology of inflammation. The objectives of this study were to determine the effect of M. peregrina seed ethanolic extract (MPSE) on the viability of and NO and IL-1β production by lipopolysaccharide (LPS)-activated macrophage (J774A.1) cell line. Materials and Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay was used to determine the cytotoxic effect of MPSE treatment at concentrations ranging from 31.15 to 1000 μg/mL. The NO concentration was determined by Griess assay and IL-1β proinflammatory cytokine concentration by enzyme-linked immunosorbent assay in the supernatant of MPSE-treated LPS-activated J774A.1 cell culture. Results: The results show that the MPSE was not cytotoxic at 1000 μg/mL but significantly (p |
| Databáze: | OpenAIRE |
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