Investigation of sex-specific effects of apolipoprotein E on severity of EAE and MS

Autor: Schrewe, L., Lill, C. M., Liu, T., Salmen, A., Gerdes, L. A., Guillot-Noel, L., Akkad, D. A., Blaschke, P., Graetz, C., Hoffjan, S., Kroner, A., Demir, S., Böhme, A., Rieckmann, P., Elali, A., Hagemann, N., Hermann, D. M., Cournu-Rebeix, I., Zipp, F., Kümpfel, T., Buttmann, M., Zettl, U. K., Fontaine, B., Bertram, L., Gold, R., Chan, A.
Přispěvatelé: St. Josef Hospital, Ruhr-University Bochum, Max-Planck-Institut für Molekulare Genetik (MPIMG), Max-Planck-Gesellschaft, Platform for Genome Analytics, University Medical Center of the Johannes Gutenberg University Mainz, Max Planck Institute for Human Development, Institute for Clinical Neuroimmunology, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Department of Human Genetics, Ruhr-Universität Bochum [Bochum], Department of Neurology, McGill University Health Center [Montreal] (MUHC), Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Neuroscience Axis, Research Center of CHU de Québec—CHUL, Universität Duisburg-Essen [Essen], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imperial College of Science, Technology and Medicine (IMPERIAL COLLEGE), Queen Mary University of London (QMUL)-Imperial College of Science, Technology and Medicine, HAL-UPMC, Gestionnaire, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Rostock, Julius-Maximilians-Universität Würzburg (JMU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Universität Duisburg-Essen = University of Duisburg-Essen [Essen]
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Encephalomyelitis
Autoimmune
Experimental
Genotype
Immunology
Medizin
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Multiple sclerosis
Cellular and Molecular Neuroscience
Mice
apoE
Apolipoproteins E
Sex Factors
INFLAMMATION
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
ddc:610
ALZHEIMER-DISEASE
Mice
Knockout
Science & Technology
Neurology & Neurosurgery
Research
Neurosciences
Gender
CNS DISEASE
1103 Clinical Sciences
MULTIPLE-SCLEROSIS
ASSOCIATION
COGNITIVE FUNCTION
Mice
Inbred C57BL
Neurology
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
1107 Immunology
DISEASE SEVERITY
IMMUNE-SYSTEM
Female
lipids (amino acids
peptides
and proteins)
MSSS
Association studies in genetics
Neurosciences & Neurology
1109 Neurosciences
Life Sciences & Biomedicine
Zdroj: Journal of Neuroinflammation
Journal of Neuroinflammation, BioMed Central, 2015, 12 (1), pp.234. ⟨10.1186/s12974-015-0429-y⟩
Journal of Neuroinflammation, 2015, 12 (1), pp.234. ⟨10.1186/s12974-015-0429-y⟩
ISSN: 1742-2094
Popis: Background Despite pleiotropic immunomodulatory effects of apolipoprotein E (apoE) in vitro, its effects on the clinical course of experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS) are still controversial. As sex hormones modify immunomodulatory apoE functions, they may explain contentious findings. This study aimed to investigate sex-specific effects of apoE on disease course of EAE and MS. Methods MOG35-55 induced EAE in female and male apoE-deficient mice was assessed clinically and histopathologically. apoE expression was investigated by qPCR. The association of the MS severity score (MSSS) and APOE rs429358 and rs7412 was assessed across 3237 MS patients using linear regression analyses. Results EAE disease course was slightly attenuated in male apoE-deficient (apoE−/−) mice compared to wildtype mice (cumulative median score: apoE−/− = 2 [IQR 0.0–4.5]; wildtype = 4 [IQR 1.0–5.0]; n = 10 each group, p = 0.0002). In contrast, EAE was more severe in female apoE−/− mice compared to wildtype mice (cumulative median score: apoE−/− = 3 [IQR 2.0–4.5]; wildtype = 3 [IQR 0.0–4.0]; n = 10, p = 0.003). In wildtype animals, apoE expression during the chronic EAE phase was increased in both females and males (in comparison to naïve animals; p
Databáze: OpenAIRE
Externí odkaz: