Application of polymerase chain reaction (PCR) to the microscopically identified cells on the slides: evaluation of specificity and sensitivity of single cell PCR
| Autor: | N, Teramoto, Y, Tonoyama, T, Akagi, A B, Sarker, T, Yoshino, I, Yamadori, K, Takahashi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Human T-lymphotropic virus 1
Paraffin Embedding paraffin section polymerase chain reaction human T-lymphotropic virus type I Genome Viral single copy gene Immunohistochemistry Sensitivity and Specificity Salivary Glands Globins single cell Blotting Southern DNA Viral Tumor Cells Cultured Humans Leukemia-Lymphoma Adult T-Cell Cell Line Transformed |
| Zdroj: | Acta Medica Okayama. 48(4):189-193 |
| ISSN: | 0386-300X |
| Popis: | The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells. |
| Databáze: | OpenAIRE |
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