Antioxidant activity and phenolic composition in Pomegranate (Punica granatum, L.) genotypes from south Italy by UHPLC/Orbitrap-MS approach
| Autor: | Vita, Di Stefano, Rosa, Pitonzo, Maria Eugenia, Novara, David, Bongiorno, Serena, Indelicato, Carla, Gentile, Giuseppe, Avellone, Rosaria, Bognanni, Salvatore, Scandurra, Maria Grazia, Melilli |
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| Přispěvatelé: | Di Stefano, Vita, Pitonzo, Rosa, Novara, Maria Eugenia, Bongiorno, David, Indelicato, Serena, Gentile, Carla, Avellone, Giuseppe, Bognanni, Rosaria, Scandurra, Salvatore, Melilli, Maria Grazia |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Lythraceae
phenolic compound Pomegranate genotypes Nutrition and Dietetics Genotype Settore CHIM/10 - Chimica Degli Alimenti antioxidant activity pomegranate genotype phenolic compounds anthocyanins Antioxidants anthocyanin Fruit and Vegetable Juices UHPLC/Orbitrap-MS UHPLC–Orbitrap-MS Italy Phenols Tandem Mass Spectrometry Fruit Settore BIO/10 - Biochimica Settore CHIM/01 - Chimica Analitica Agronomy and Crop Science Chromatography High Pressure Liquid Biotechnology Food Science |
| Zdroj: | Journal of the science of food and agriculture (Online) (2018). doi:10.1002/jsfa.9270 info:cnr-pdr/source/autori:Vita Di Stefano1*, Rosa Pitonzo2, Maria Eugenia Novara2, David Bongiorno1, Serena Indelicato1, Carla Gentile1, Giuseppe Avellone1, Rosaria Bognanni3, Salvatore Scandurra3, Maria Grazia Melilli3./titolo:Antioxidant activity and phenolic composition in Pomegranate (Punica granatum, L.) genotypes from south Italy by UHPLC%2FOrbitrap-MS approach/doi:10.1002%2Fjsfa.9270/rivista:Journal of the science of food and agriculture (Online)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.1002/jsfa.9270 |
| Popis: | Background: Pomegranate fruits are a rich source of polyphenols with numerous health-promoting effects. Pomegranate juices of five genotypes (‘Mollar’, ‘Kingdom’, ‘Dente di Cavallo’, and two old populations ‘Francofonte’ and ‘Santa Tecla’) were evaluated regarding anthocyanin and non-anthocyanin phenolic contents using ultrahigh performance liquid chromatography (UHPLC)–Orbitrap-mass spectrometry (MS). Moreover, total antioxidant activity (TAA) was evaluated using a 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay. Results: Twenty-three phenolic compounds were identified. Cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside were the most representative anthocyanins in all genotypes; the Santa Tecla population had the highest content of these anthocyanins, 97.64 mg L−1 and 40.29 mg L−1 respectively. In the Francofonte population, ferulic acid hexoside was the most abundant compound (391.18 mg L−1). TAA values ranged between 221.5 and 36.73 µmol Trolox equivalents/100 mL of juice. A high TAA value was recorded for the Santa Tecla pomegranate population. Conclusion: The UHPLC–Orbitrap-MS approach was employed for the first time to identify the phenolic compound profiling in five pomegranate genotypes. TAA was analysed using an ABTS assay, and the results showed a significant variability in nutraceutical potential of the pomegranate genotypes studied. The inclusion of phenolic information in the linear discriminant analysis allowed very good discriminations among genotypes to be obtained. © 2018 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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