Presence of a very small population of Thy-1+, L3T4+ cells producing large amounts of IL-3 in young athymic nude mice
| Autor: | Kimoto, M., de Kossodo, S., Kindler, V., Detraz, M., Vassalli, Pierre, Izui, Shozo |
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| Rok vydání: | 1989 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Antigens Differentiation T-Lymphocyte/ analysis Mice Inbred BALB C Ionomycin Lymphocytes/immunology/ metabolism Mice Nude ddc:616.07 Lymphocyte Activation Mice Interleukin-3/ metabolism Antigens Surface Animals Tetradecanoylphorbol Acetate Thy-1 Antigens Interleukin-3 Lymphocytes Antigens Surface/ analysis Antigens Thy-1 Cells Cultured Research Article |
| Zdroj: | Immunology, Vol. 68, No 4 (1989) pp. 557-563 |
| ISSN: | 0019-2805 |
| Popis: | A mixture of phorbol myristate acetate (PMA) and ionomycin was found to stimulate spleen and lymph node cells (LNC) from 6 to 8 week-old-athymic BALB/c nude mice, as well as from control +/+ mice, to secrete interleukin-3 (IL-3) in vitro. The specificity of the IL-3 bioassay was attested to by the use of rabbit anti-IL-3 antibodies, and by the detection of an accumulation of IL-3 mRNA. Cytotoxic treatment with relevant antibodies showed that the cells responsible for the IL-3 production in athymic nude mice was Thy-1+, L3T4+, Ly2-, while both L3T4+ and Ly 2+ cells produced IL-3 in control +/+ mice. Although the levels of IL-3 secreted by nude LNC varied among experiments, nude LNC were able to produce IL-3 at a level comparable to or higher than +/+ LNC. In addition, nude LNC consistently secreted two to three times more granulocyte-macrophage colony-stimulating factor (GM-CSF) than +/+ LNC, and the majority of GM-CSF secretion was dependent on the presence of L3T4+ cells. In contrast, IL-2 production by nude LNC was markedly limited. Since the flow microfluorometry analysis failed to demonstrate the presence of L3T4+ cells (less than 1%) in nude LNC, compared with 40-50% L3T4+ cells in +/+ LNC, our results suggest that athymic nude mice have a small population of Thy-1+, L3T4+ cells characterized by its ability to secrete IL-3 and GM-CSF at a very high rate. |
| Databáze: | OpenAIRE |
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