Genomic structure of the human pre-T cell receptor alpha chain and expression of two mRNA isoforms

Autor: Saint-Ruf, Claude, Lechner, Oskar, Feinberg, Jacqueline, Von Boehmer, Harald
Přispěvatelé: Développement du système immunitaire (Inserm U373), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Oskar Lechner is a recipient of a Marie Curie Training Grant from the European Comission. Harald von Boehmer is supported by the Institut Universitaire de France, by the Juvenile Diabetes Foundation (USA), and by the Koerber Foundation (Germany). This work was supported in part by the Institut National de la Santé et de la Recherche Médicale, Paris, and by the Faculté Necker Enfants Malades, Descartes Université, Paris., Saint-Ruf, Claude
Jazyk: angličtina
Rok vydání: 1998
Předmět:
Zdroj: European Journal of Immunology
European Journal of Immunology, Wiley-VCH Verlag, 1998, 28 (11), pp.3824-31. ⟨10.1002/(SICI)1521-4141(199811)28:113.0.CO;2-9⟩
ISSN: 0014-2980
1521-4141
DOI: 10.1002/(SICI)1521-4141(199811)28:113.0.CO;2-9⟩
Popis: International audience; The pre-TCR, which is minimally composed of the TCR g chain, the pre-T § chain, and the CD3 complex, regulates early T cell development. The pre-T § chain is a 33-kDa type I trans-membrane glycoprotein with an extracellular part similar to the constant domain of the immunoglobulin supergene family. We have sequenced (11 kb) the human pT § gene, which like the murine pT § gene consists of four exons: exon 1 encodes the 5' untranslated region, the leader peptide and the first three amino acids of the mature protein, exon 2 the extracel-lular immunoglobulin (Ig)-like domain, exon 3 a 15-amino acid peptide including a cysteine required for heterodimerization with TCR g , exon 4 the transmembrane region, the cytoplas-mic tail and the 3' untranslated sequence. The human pT § gene is located on chromosome 6p21.3, close to the HLA-A locus. Reverse transcription-PCR studies with human thymus and leukemic cells showed that alternative splicing produces a shorter pT § isoform, which lacks the Ig-like domain but contains the transmembrane elements and the extracytoplas-mic cystein and which could thus permit pairing with TCR g chain and association with CD3 molecules. The conserved splice sites suggest a yet ill-defined biological function of the short pT § protein.
Databáze: OpenAIRE
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