Endotracheal intubation in mice via direct laryngoscopy using an otoscope
| Autor: | Thomas, Joanna L., Dumouchel, Justin, Li, Jinghong, Magat, Jenna, Balitzer, Dana, Bigby, Timothy D. |
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| Rok vydání: | 2014 |
| Předmět: |
Assistive Technology
lung physiology Laryngoscopy Animal intubation technique Bioengineering Mice Intratracheal Otoscopes Rare Diseases airway resistance Models Models Animal Intubation Intratracheal Respiratory Animals Medicine Psychology Cognitive Sciences Biochemistry and Cell Biology Intubation Lung Issue 86 endotracheal intubation |
| Popis: | Mice, both wildtype and transgenic, are the principal mammalian model in biomedical research currently. Intubation and mechanical ventilation are necessary for whole animal experiments that require surgery under deep anesthesia or measurements of lung function. Tracheostomy has been the standard for intubating the airway in these mice to allow mechanical ventilation. Orotracheal intubation has been reported but has not been successfully used in many studies because of the substantial technical difficulty or a requirement for highly specialized and expensive equipment. Here we report a technique of direct laryngoscopy using an otoscope fitted with a 2.0 mm speculum and using a 20 Gintravenous catheter as an endotracheal tube. We have used this technique extensively and reliably to intubate and conduct accurate assessments of lung function in mice. This technique has proven safe, with essentially no animal loss in experienced hands. Moreover, this technique can be used for repeated studies of mice in chronic models. |
| Databáze: | OpenAIRE |
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