[Contribution of the real time PCR in antenatal diagnosis of congenital toxoplasmosis]

Autor: Siala, E., Ben Abdallah, R., Delabesse, E., Aoun, K., Paris, L., Bouratbine, A.
Přispěvatelé: Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Jazyk: francouzština
Rok vydání: 2007
Předmět:
MESH: Fetal Diseases/parasitology
MESH: Polymerase Chain Reaction/methods
[SDV]Life Sciences [q-bio]
Antibody Affinity
Antibodies
Protozoan
MESH: Toxoplasma/classification
Polymerase Chain Reaction
MESH: Immunoglobulin M/blood
Toxoplasmosis
Congenital
Ultrasonography
Prenatal
MESH: Fetal Blood/immunology
MESH: Pregnancy
Pregnancy
Prenatal Diagnosis
Animals
Humans
MESH: Toxoplasmosis
Congenital/diagnostic imaging
MESH: Animals
MESH: Antibodies
Protozoan/blood
MESH: DNA
Protozoan/analysis
MESH: Humans
MESH: Infant
Newborn
Infant
Newborn
MESH: Ultrasonography
Prenatal
MESH: Follow-Up Studies
DNA
Protozoan
Amniotic Fluid
Fetal Blood
MESH: Antibody Affinity/immunology
MESH: Fetal Diseases/diagnostic imaging
Fetal Diseases
Immunoglobulin M
Immunoglobulin G
MESH: Toxoplasma/immunology
MESH: Toxoplasmosis
Congenital/diagnosis
MESH: Fetal Diseases/diagnosis
Female
MESH: Amniotic Fluid/parasitology
MESH: Prenatal Diagnosis
MESH: Immunoglobulin G/blood
Toxoplasma
MESH: Female
Follow-Up Studies
Zdroj: Tunisie Medicale
Tunisie Medicale, Maghreb-Editions; 1999, 2007, 85 (5), pp.385-8
Tunisie Medicale, 2007, 85 (5), pp.385-8
ISSN: 0041-4131
Popis: BACKGROUND: The antenatal diagnosis of congenital toxoplasmosis rests in Tunisia on ultrasonography coupled with biological explorations. Among these explorations the search of Toxoplasma gondii by means of real time PCR in amniotic fluid is the examination of choice. AIM: We report the results of 33 parturients for which the biological examinations allowed to retain the notion of perigravidic or pergravidic toxoplasmic infection. METHODS: They were 13 patients having a seroconversion during the pregnancy, 19 having anti-toxoplasmic IgM with a low or intermediate index of avidity and a patient having presented a symptomatic anteconceptional primary infection. The ADN was extracted by means of the Kit (Qiagen). Genic amplification by PCR TaqMan targeted a portion of 71 pairs of bases of the B 1 gene. RESULTS: The PCR was positive among 9 patients (27.3%). They were a patient having presented a symptomatic toxoplasmosis during the pregnancy, 4 patients having consulted only in the 2nd quarter and for which the index of avidity was intermediate and 4 patients having presented seoconversions of 1st (n=1) of 2nd (n=2) or 3rd trimester. Among these patients, 2 had a medical interruption of pregnancy. The 7 others were put under pyrimethamine sulfadiazine. The neonatal assessment practised at 5 new-born babies was negative in all the cases. The PCR was negative for 24 patients. 18 pregnancies were followed. The neonatal serology was negative. The follow-up of 13 newborn child showed the disappearance of the antitoxoplasmic IgG between the 6th and 12th month.
Databáze: OpenAIRE
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