Blending of diblock and triblock copolypeptide amphiphiles yields cell penetrating vesicles with low toxicity
| Autor: | Rodriguez, April R., Choe, Uh-Joo, Kamei, Daniel T., Deming, Timothy J. |
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| Rok vydání: | 2015 |
| Předmět: |
polypeptide
Polymers Biomedical Engineering Bioengineering Cell-Penetrating Peptides Article Macromolecular and Materials Chemistry Surface-Active Agents Methionine Spectroscopy Fourier Transform Infrared Humans Transport Vesicles Spectroscopy vesicle Drug Carriers Microscopy Chromatography Liquid Gel Microscopy Confocal copolymer Molecular Structure Chemical Engineering Flow Cytometry Nanomedicine Fourier Transform Infrared Hela Cells Confocal Chromatography Gel blends Peptides Chromatography Liquid HeLa Cells |
| Zdroj: | Macromolecular bioscience, vol 15, iss 1 Rodriguez, AR; Choe, UJ; Kamei, DT; & Deming, TJ. (2015). Blending of diblock and triblock copolypeptide amphiphiles yields cell penetrating vesicles with low toxicity. Macromolecular Bioscience, 15(1), 90-97. doi: 10.1002/mabi.201400348. UCLA: Retrieved from: http://www.escholarship.org/uc/item/6j66c30x |
| DOI: | 10.1002/mabi.201400348. |
| Popis: | We have prepared dual hydrophilic triblock copolypeptide vesicles that form both micron and nanometer scale vesicles in aqueous media. The incorporation of terminal homoarginine segments into methionine sulfoxide based vesicles was found to significantly enhance their cellular uptake compared to a diblock copolypeptide vesicle control. We also demonstrated that diblock and triblock copolypeptides with similar hydrophobic domains were found to mix well and form vesicle populations with reasonably uniform compositions. Blending of diblock and triblock copolypeptide amphiphiles in vesicle nanocarriers was found to impart these materials with many advantageous properties, including good cellular uptake while maintaining minimal toxicity, as well as capability for biological responsiveness to promote vesicle disruption and release of encapsulated cargos. |
| Databáze: | OpenAIRE |
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