Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand
| Autor: | Morteza Yaftian, Fatemeh Yari, Mehran Ghasemzadeh, Vahid Fallah Azad, Mansoureh Haghighi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Cell Journal (Yakhteh) Cell Journal, Vol 20, Iss 1, Pp 120-126 (2018) |
| ISSN: | 2228-5806 |
| Popis: | Objective The in vitro treatment of tumor cells with platelet (Plt) causes inhibition of tumor cell growth, although mechanism of this effect is not clear yet. Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells. The purpose of this study was to clarify the role of Plts and Plt-derived components in the induction of apoptosis in the blood mononuclear cells of patients with leukemia. Materials and Methods In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with Plts, Plt-derived microparticles (Plt-MPs) as well as purified soluble CD40L (sCD40L). After 48 hours of co-culture, the anti-cancer activity of the aforementioned factors was surveyed using examination of apoptosis markers of the cells including active caspase-3 and CD95 using ELISA and flow cytometer techniques, respectively. Additionally, staining of the cells with 7-Aminoactinomycin D (7-AAD) was evaluated by flow cytometer technique. Trypan blue exclusion test and WST-1 method were also used to compare the death/survival status of the cells. Results Levels of CD95 and caspase-3 were significantly increased in the all treated groups (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|