Biodistribution, Stability, and Blood Distribution of the Cell Penetrating Peptide Maurocalcine in Mice
| Autor: | Perret, Pascale, Ahmadi, Mitra, Riou, Laurent, Bacot, Sandrine, Pecher, Julien, Poillot, Cathy, Broisat, Alexis, Ghezzi, Catherine, De Waard, Michel |
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| Přispěvatelé: | Radiopharmaceutiques biocliniques (LRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Smartox Biotechnologies [Saint Martin d’Hères, France] (Bâtiment Nanobio), Université Joseph Fourier - Grenoble 1 (UJF)-FLORALIS, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was partly funded by the French program 'Investissements d’Avenir run by the 'Agence Nationale pour la Recherche', grant 'Infrastructure d’avenir en Biologie et Santé—ANR-11-INBS-0006'. MDW is supported by a LabEx ANR grant N ANR-11-LABX-0015., ANR-11-INBS-0006,FLI,France Life Imaging(2011), ANR-11-IDEX-0005,USPC,Université Sorbonne Paris Cité(2011), Perret, Pascale, Infrastructures - France Life Imaging - - FLI2011 - ANR-11-INBS-0006 - INBS - VALID, Université Sorbonne Paris Cité - - USPC2011 - ANR-11-IDEX-0005 - IDEX - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Scorpion Venoms
blood stability Cell-Penetrating Peptides [SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine Article [SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine lcsh:Chemistry Mice Drug Stability Animals Tissue Distribution cardiovascular diseases lcsh:QH301-705.5 Chromatography High Pressure Liquid X-Ray Microtomography nervous system diseases maurocalcine lcsh:Biology (General) lcsh:QD1-999 Isotope Labeling Positron-Emission Tomography drug delivery in vivo biodistribution cardiovascular system cell-penetrating peptide circulatory and respiratory physiology |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2015, 16 (11), pp.27730-27740. ⟨10.3390/ijms161126054⟩ International Journal of Molecular Sciences, Vol 16, Iss 11, Pp 27730-27740 (2015) Volume 16 Issue 11 Pages 27730-27740 International Journal of Molecular Sciences, 2015, 16 (11), pp.27730-27740. ⟨10.3390/ijms161126054⟩ |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms161126054⟩ |
| Popis: | International audience; Maurocalcine (MCa) is the first natural cell penetrating peptide to be discovered in animal venom. In addition to the fact that it represents a potent vector for the cell penetration of structurally diverse therapeutic compounds, MCa also displays several distinguishing features that make it a potential peptide of choice for clinical and biotechnological applications. The aim of the present study was to gain new information about the properties of MCa in vivo in order to delineate the future potential applications of this vector. For this purpose, two analogues of this peptide with (Tyr-MCa) and without (Lin-Tyr-MCa) disulfide bridges were synthesized, radiolabeled with (125)I, and their in vitro stabilities were first evaluated in mouse blood. The results indicated that (125)I-Tyr-MCa was stable in vitro and that the disulfide bridges conferred a competitive advantage for the stability of peptide. Following in vivo injection in mice, (125)I-Tyr-MCa targeted peripheral organs with interesting quantitative differences and the main route of peptide elimination was renal. |
| Databáze: | OpenAIRE |
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