Fifty-nine bp repeat polymorphism in the uncommon intron 36 of the human mucin gene MUC5B

Autor: J L, Desseyn, K, Rousseau, A, Laine
Přispěvatelé: Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Biologie et Physiopathologie des Cellules Mucipares (u377), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), This work was supported by Le Comitÿ du Nord de LaLigue contre le Cancer and L'Association de Recherche sur le Cancer. Jean-Luc Desseyn was supported by a fellowship from the Ministère de l'Education Supérieure et de la Recherche., The authors wish to thank Annette Leclercq and Christine Mouton for their technical assistance. We also thank Pascal Mathon for performing the automated DNA sequencing., Lille Inflammation Research International Center - U 995 [LIRIC]
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Zdroj: Electrophoresis
Electrophoresis, Wiley-VCH Verlag, 1999, 20 (3), pp.493-496. ⟨10.1002/(SICI)1522-2683(19990301)20:33.0.CO;2-7⟩
Electrophoresis, 1999, 20 (3), pp.493-496. ⟨10.1002/(SICI)1522-2683(19990301)20:33.0.CO;2-7⟩
ISSN: 0173-0835
1522-2683
DOI: 10.1002/(SICI)1522-2683(19990301)20:33.0.CO;2-7⟩
Popis: International audience; A variable number of tandem repeat (VNTR) polymorphism within the intron 36 of the human mucin gene MUC5B, which is mapped to chromosome 11 band p15.5, have been identified using Southern blotting experiments. This polymorphism can be easily assayed by polymerase chain reaction (PCR) to detect linkage of inherited disorder. Five alleles were observed in 86 unrelated individuals due to 3-8 direct perfect repeats of 59 bp. This repeat has the particularity to begin at the end of the preceding exon. Southern blot experiments revealed the locus specificity of the repeat. The sequence of the repeat unit does not match the consensus sequence of Chi-related minisatellites.
Databáze: OpenAIRE
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