| Autor: |
Am, Weissman, Ross P, Et, Luong, Garcia-Morales P, Ml, Jelachich, We, Biddison, Rd, Klausner, Le, Samelson |
| Rok vydání: |
1988 |
| Předmět: |
|
| Zdroj: |
Europe PubMed Central |
| ISSN: |
0022-1767 |
| Popis: |
TCR stimulation by Ag or anti-receptor antibodies in murine T cells results in the activation of two independent protein kinases, protein kinase C (PKC) and a protein tyrosine kinase. Similarly, stimulation of murine Thy-1 or Ly-6 with mAb also results in activation of both of these kinase pathways. Tyrosine phosphorylation in all cases occurs on the TCR zeta-chain. It is known that Ag and anti-receptor antibodies activate PKC in human T cells. In this study we demonstrate that mitogen or anti-CD3 antibodies activate tyrosine phosphorylation of the human TCR-zeta-chain. PMA, which activates PKC, does not result in zeta-chain tyrosine phosphorylation. Stimulation of human T cells by antibodies that bind the CD2 molecule is an alternate mode of inducing T cell proliferation. These antibodies surprisingly do not induce tyrosine phosphorylation of the zeta-chain. Thus, different methods of cellular activation can result in distinguishable patterns of receptor-mediated biochemical signaling events. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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