3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
| Autor: | Ribot, Emeline J., Tournier, Clement, Aid-Launais, Rachida, Koonjoo, Neha, Oliveira, Hugo, Trotier, Aurelien J., Rey, Sylvie, Wecker, Didier, Letourneur, Didier, Amedee Vilamitjana, Joelle, Miraux, Sylvain |
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| Přispěvatelé: | Centre de résonance magnétique des systèmes biologiques (CRMSB), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bruker BioSpin MRI GmbH [Ettlingen, Germany], VIAUD, Karine, Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Bone Regeneration
Tissue Engineering [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Science Biocompatible Materials X-Ray Microtomography Immunohistochemistry Article Rats Disease Models Animal Imaging Three-Dimensional [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging Medicine Animals Female Femur Biomarkers Magnetic Resonance Angiography |
| Zdroj: | Scientific Reports Scientific Reports, 2017, 7 (1), pp.6100. ⟨10.1038/s41598-017-06258-0⟩ Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.6100. ⟨10.1038/s41598-017-06258-0⟩ Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-06258-0⟩ |
| Popis: | International audience; Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam. |
| Databáze: | OpenAIRE |
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