Characterization of human and mouse c-fes cDNA clones and identification of the 5' end of the gene
| Autor: | Myriam Alcalay, Antolini F, Wj, Ven, Lanfrancone L, Grignani F, Pg, Pelicci |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Base Sequence
Placenta Molecular Sequence Data Restriction Mapping DNA Exons Protein-Tyrosine Kinases Methylation Cell Line Mice Proto-Oncogene Proteins c-fes Pregnancy Proto-Oncogene Proteins Sequence Homology Nucleic Acid Proto-Oncogenes Animals Humans Female Amino Acid Sequence RNA Messenger Gene Library |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 0950-9232 |
| Popis: | cDNA clones of the human c-fes mRNA were isolated. Nucleotide analysis showed that c-fes mRNA contains a 2514 nucleotide open reading frame, which could encode for a 93 kDa protein, and both 5' and 3' v-fes nonhomologous sequences. Primer extension experiments confirmed that the longest isolated cDNAs are about the same length as the entire human c-fes mRNA. Sequence comparison between human c-fes cDNA and the corresponding genomic regions identified a 5' viral-non homologous exon (exon 1) located 491 bp upstream from the first v-fes homologous exon. The genomic region surrounding c-fes exon 1 contains a CpG island and acts as a promoter in vitro. Analysis of the 5' end of mouse c-fes cDNA suggested that the 5' human and mouse gene structure are similar. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|