Defining Metaniches in the Oral Cavity According to Their Microbial Composition and Cytokine Profile
| Autor: | Seidel, Corinna L., Gerlach, Roman G., Wiedemann, Patrick, Weider, Matthias, Rodrian, Gabriele, Hader, Michael, Frey, Benjamin, Gaipl, Udo S., Bozec, Aline, Cieplik, Fabian, Kirschneck, Christian, Bogdan, Christian, Gölz, Lina |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
DNA Bacterial Male multiplex immunoassay oral niches cytokine profile Article lcsh:Chemistry Young Adult Tongue oral biofilms and fluids RNA Ribosomal 16S Humans ddc:610 Saliva lcsh:QH301-705.5 Mouth Palate Microbiota Gingival Crevicular Fluid oral microbiota metaniches stomatognathic diseases lcsh:Biology (General) lcsh:QD1-999 Cytokines next-generation sequencing Female |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 8218, p 8218 (2020) Volume 21 Issue 21 |
| ISSN: | 1422-0067 |
| Popis: | The human oral microbiota consists of over 700 widespread taxa colonizing the oral cavity in several anatomically diverse oral niches. Lately, sequencing of the 16S rRNA genes has become an acknowledged, culture-independent method to characterize the oral microbiota. However, only a small amount of data are available concerning microbial differences between oral niches in periodontal health and disease. In the context of periodontitis, the cytokine expression in the gingival crevicular fluid has been studied in detail, whereas little is known about the cytokine profile in hard and soft tissue biofilms. In order to characterize oral niches in periodontal health, the oral microbiota and cytokine pattern were analyzed at seven different sites (plaque (P), gingival crevicular fluid (GCF), saliva (S), tongue (T), hard palate (HP), cheek (C) and sublingual area (U)) of 20 young adults using next-generation sequencing and multiplex immunoassays. Site-specific microbial compositions were detected, which clustered into three distinct metaniches (&ldquo P-GCF&rdquo &ldquo S-T-HP&rdquo and &ldquo C-U&rdquo ) and were associated with niche-/metaniche-specific cytokine profiles. Our findings allow the definition of distinct metaniches according to their microbial composition, partly reflected by their cytokine profile, and provide new insights into microenvironmental similarities between anatomical diverse oral niches. |
| Databáze: | OpenAIRE |
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