Interleukin-7 is a potent co-stimulus of the adhesion pathway involving CD2 and CD28 molecules
| Autor: | Régis Costello, Brailly H, Mallet F, Mawas C, Olive D |
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| Rok vydání: | 1993 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes CD8 Antigens Interleukin-7 T-Lymphocytes CD2 Antigens hemic and immune systems chemical and pharmacologic phenomena Receptors Interleukin-2 Lymphocyte Activation Recombinant Proteins CD28 Antigens Antigens CD Humans Interleukin-2 Receptors Immunologic Cell Division Cells Cultured Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0019-2805 |
| Popis: | Co-stimulation of highly purified peripheral T lymphocytes from healthy blood donors with the adhesion molecules CD2 and CD28 in association with recombinant interleukin-7 (rIL-7) induced T-cell proliferation, multiple cytokine secretion and IL-2 receptivity. We demonstrated that rIL-7 is as potent as rIL-2 in inducing the proliferation of unseparated, CD4+ and CD8+ T cells. In contrast to low or undetectable levels of IL-1 alpha, IL-6 and IL-2, high levels of tumour necrosis factor-alpha (TNF-alpha), IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were secreted. Experiments using blocking antibodies suggested a direct mechanism for rIL-7 co-stimulatory effect, although induction of the CD25/IL-2 receptor alpha-chain (CD25/IL-2R alpha) was observed. Monoclonal antibodies (mAb) against the adhesion molecules CD2 and CD28 are likely to mimic the interaction with their respective physiological ligands [lymphocyte function-associated antigen-3 (LFA-3)/CD58, CD59 and CD48 for CD2, B7/BB1 for CD28]. Taken together, these in vitro data suggest that IL-7 could participate in paracrine interactions between T lymphocytes and thymic stromal cells or dendritic cells, via its potent co-stimulatory activity with CD2 and CD28 adhesion molecules. |
| Databáze: | OpenAIRE |
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