Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter)
| Autor: | Wawrosch, Christoph, Schwaiger, Stefan, Stuppner, Hermann, Kopp, Brigitte |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
MS nutrient medium after Murashige and Skoog (1962) Hairy roots Lignan Agrobacterium Cyclopentanes Acetates Asteraceae YE yeast extract 5-Methoxy-leoligin Elicitor Agrobacterium rhizogenes Plant Roots Article Leontopodium nivale ssp. alpinum Leoligin Lignans MLG 5-methoxy-leoligin MeJa methyl jasmonate Edelweiss Culture Techniques Drug Discovery LG leoligin Oxylipins |
| Zdroj: | Fitoterapia |
| ISSN: | 1873-6971 0367-326X |
| Popis: | A hairy root line of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter) was obtained upon transformation with Agrobacterium rhizogenes strain ATCC15834. Elicitation of this line with silver nitrate, sucrose, methyl jasmonate and yeast extract at various concentrations in most cases resulted in a stimulation of lignan biosynthesis. Through elicitation with 6% sucrose the roots accumulated the pharmacologically active lignans leoligin and 5-methoxy-leoligin at levels of 0.0678% and 0.0372%, respectively, without significant growth inhibition. These lignan levels were comparable to those found in intact roots of cultivated Edelweiss. The biotechnological production of leoligin could be an attractive option for the continuous, field culture-independent production of the valuable secondary metabolites leoligin and 5-methoxy-leoligin. Graphical abstract |
| Databáze: | OpenAIRE |
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