Characterisation of alpha 1A Ba2+, Sr2+ and Ca2+ currents recorded with the ancillary beta 1-4 subunits
| Autor: | Mangoni, Me, Thierry Cens, Dalle, C., Nargeot, J., Charnet, P. |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Europe PubMed Central ResearcherID |
| ISSN: | 1060-6823 |
| Popis: | Xenopus oocytes have been injected with different combinations of expression plasmids carrying the rat brain alpha 1A and different beta (beta 1-4) Ca2+ channel subunit cDNAs. Whole-cell Ba2+ and Ca2+ currents were recorded up to seven days after injection. Intra-oocyte injection of BAPTA allowed us to record uncontaminated Ba2+, Sr2+ currents. The alpha 1A calcium channel showed relative current amplitudes according to the sequence: IBa2+ISr2+ICa2+. The ratio ICa2+/IBa2+ was significantly larger when compared to the class C L-type Ca2+ channel (alpha 1C). However, currents flowing through alpha 1A and alpha (1C) subunits saturate for similar Ba2+ concentrations and display the anomalous mole fraction effect in the presence of mixtures of Ba2+ and Ca2+ ions in the external medium. In oocytes expressing the alpha 1A Ca2+ channel subunit, switching from extracellular Ba2+ to Ca2+ also induced a depolarising shift of current-to-voltage relation and the steady-state inactivation curve, and increased the time-to-peak of the current. Inactivation kinetics were poorly affected. Changes in gating and voltage-dependence of activation, but not in the voltage-dependent inactivation, were independent from the coexpressed beta subunit (except with the beta 4 subunit). Our data constitute strong evidence for the existence of differences in intra-pore Ca2+ binding sites between the alpha 1C and alpha 1A subunits, and emphasise the influence of the charge carrier on the modulation of alpha 1A properties by the beta subunits. |
| Databáze: | OpenAIRE |
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