| Autor: |
Lempereur, Laurence, Brambilla, Daria, Maria Scoto, Giovanna, D'Alcamo, Maria, Goffin, Vincent, Crosta, Lucia, Palmucci, Tullio, Rampello, Liborio, Bernardini, Renato, Cantarella, Giuseppina |
| Jazyk: |
angličtina |
| Rok vydání: |
2003 |
| Předmět: |
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| Popis: |
1. Undesired effects of cancer radiotherapy mainly affect the hematopoietic system. Growth hormone (GH) participates in both hematopoiesis and modulation of the immune response. We report both r-hGH cell death prevention and restoration of secretory capacities of irradiated human peripheral blood lymphocytes (PBL) in vitro. 2. r-hGH induced cell survival and increased proliferation of irradiated cells. Western blot analysis indicated that these effects of GH were paralleled by increased expression of the antiapoptotic protein Bcl-2. 3. r-hGH restored mitogen-stimulated release of IL-2 by PBL. Preincubation of irradiated lymphocytes with the growth hormone receptor (GHR) antagonists B2036 and G120 K abrogated r-hGH-dependent IL-2 release. 4. These results demonstrate that r-hGH protects irradiated PBL from death in a specific, receptor-mediated manner. Such effect of r-hGH on PBL involves activation of the antiapoptotic gene bcl-2 and prevention of cell death, associated with preserved functional cell capacity. Finally, potential use of GH as an immunopotentiating agent could be envisioned during radiation therapy of cancer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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