Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging
| Autor: | Mollink, J., Kleinnijenhuis, M., Cappellen van Walsum, A.M. van, Sotiropoulos, S.N., Cottaar, M., Mirfin, C., Heinrich, M.P., Jenkinson, M., Pallebage-Gamarallage, M., Ansorge, O., Jbabdi, S., Miller, K.L. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Aged
80 and over Male Microscopy Post-mortem Histological Techniques Polarized light imaging Tissue Banks Dispersion Middle Aged Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3] White Matter Article lcsh:RC321-571 Diffusion MRI Sir Peter Mansfield Imaging Centre (SPMIC) Diffusion Magnetic Resonance Imaging Myelin Astrocytes Validation Humans Beacon - Precision Imaging Microscopy Polarization lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Myelin Sheath Aged |
| Zdroj: | Neuroimage NeuroImage, Vol 157, Iss, Pp 561-574 (2017) NeuroImage, 157, 561-574 NeuroImage, 157, pp. 561-574 |
| ISSN: | 1095-9572 1053-8119 |
| Popis: | Contains fulltext : 177726.pdf (Publisher’s version ) (Open Access) Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain's fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology. Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region - and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract. The region-wise analysis yielded correlation coefficients of r = 0.79 for the diffusion MRI and histology comparison, while r = 0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI. These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data. |
| Databáze: | OpenAIRE |
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