Piezo-electrically driven mechanical stimulation of sensory neurons
| Autor: | Hao, Jizhe, Ruel, Jérôme, Coste, Bertrand, Roudaut, Yann, Crest, Marcel, Delmas, Patrick |
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| Přispěvatelé: | Centre de recherche en neurobiologie - neurophysiologie de Marseille ( CRN2M ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Montpellier 1 ( UM1 ), Laboratoire de neurophysiologie cellulaire ( LNPC ), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique ( CNRS ), Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1), Laboratoire de neurophysiologie cellulaire (LNPC), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Patch-Clamp Techniques [ SDV.BBM.BC ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Electricity Sensory Receptor Cells Ganglia Spinal Animals Rats Wistar [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Mechanotransduction Cellular Electrophysiological Phenomena Mechanical Phenomena Rats |
| Zdroj: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2013, 998, pp.159-70. 〈10.1007/978-1-62703-351-0_12〉 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2013, 998, pp.159-70. ⟨10.1007/978-1-62703-351-0_12⟩ Methods in Molecular Biology, 2013, 998, pp.159-70. ⟨10.1007/978-1-62703-351-0_12⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-62703-351-0_12〉 |
| Popis: | International audience; Mechanotransduction, the conversion of a mechanical stimulus into a biological response, constitutes the basis of a variety of physiological functions such as the senses of touch, balance, proprioception, blood pressure, and hearing. In vertebrates, mechanosensation is mediated by mechanosensory neurons, whose cell bodies are located in trigeminal and dorsal root ganglia. Here, we describe an in vitro model of mechanotransduction that provides an opportunity to explore the properties of mechanosensitive channels in mammalian sensory neurons. The mechano-clamp method allows applying local force on plasma membrane of whole-cell patch-clamped sensory neurons. This technique uses a mechanical probe driven by a computer-assisted piezoelectric microstage to repeatedly stimulate sensory neurons with accurate control of stimulus strength, duration, and speed. |
| Databáze: | OpenAIRE |
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