Microregional blood flow in murine and human tumours assessed using laser Doppler microprobes

Autor:	Hill, S. A., Pigott, K. H., Saunders, M. I., Powell, M. E. B., Arnold, S., Obeid, A., Ward, G., Martin Leahy, Hoskin, P. J., Chaplin, D. J.
Předmět:	Aged 80 and over Mice SCID Middle Aged Mice Regional Blood Flow Colonic Neoplasms Laser-Doppler Flowmetry Mice Inbred CBA Animals Humans sense organs Sarcoma Experimental skin and connective tissue diseases Research Article Aged
Zdroj:	Web of Science ResearcherID
Popis:	A multichannel laser Doppler system has been used to measure microregional fluctuations in perfusion in the HT29 human tumour xenograft and in patients with advanced malignant disease. A comparison is made with previously obtained data for the SaF, a transplantable murine tumour. The 300 microns diameter probes recorded fluctuations in erythrocyte flux in tumour microregions with an estimated volume of 10(-2) mm3. Of the 66 human tumour microregions sampled, 26% showed a change in erythrocyte flux by a factor of 2 or more over the 60 min measurement period, compared with 37% of HT29 and 48% of SaF microregions. In each of the studies more than 50% of changes were completed within 20 min, although slower changes were more common in the human tumours than in the experimental systems. Within the 1 h monitoring period at least 30% of the changes were reversed (human tumours 30%, HT29 45%, SaF 31%). These findings demonstrate that microregional changes in erythrocyte flux, consistent with transient, perfusion-driven changes in oxygenation, are a feature of human malignancies as well as experimental transplanted tumours.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::e12c853d86bb2a2db54300522a7df776 https://publons.com/publon/8354507/ Zobrazit plný text záznamu