Degeneration of the Nonrecombining Regions in the Mating-Type Chromosomes of the Anther-Smut Fungi
| Autor: | Fontanillas, Eric, Hood, Michael E, Badouin, Hélène, Petit, Elsa, Barbe, Valérie, Gouzy, Jerome, De Vienne, Damien, Aguileta, Gabriela, Poulain, Julie, Wincker, Patrick, Chen, Zehua, San Toh, Su, Cuomo, Christina A., Perlin, Michael, Gladieux, Pierre, Giraud, Tatiana |
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| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS), Department of Biology, Amherst College, Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Ecologie Systématique et Evolution (ESE), Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique d'Evry (IG), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), King‘s College London, Department of Biology - Program on Disease Evolution, University of Louisville, Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], Department of Biology, Program on Disease Evolution, Univ. Paris-Sud, AgroParisTech, Université Paris-Saclay, Agence Nationale de la Recherche : ANR-09-BLAN-064, European Research Council : GenomeFun 309403, National Science Foundation : DEB 0747222 0947963, ANR-09-BLAN-0064,FungiSex,Evolution des types sexuels et des chromosomes sexuels chez les champignons(2009), European Project: 309403,EC:FP7:ERC,ERC-2012-StG_20111109,GENOMEFUN(2013), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Recombination
Genetic Sex Chromosomes Silene latifolia Base Sequence Y chromosome Evolutionary strata Basidiomycota Autosomes [SDV]Life Sciences [q-bio] Molecular Sequence Data Microbotryum violaceum evolutionary strata Sequence Analysis DNA Fongs -- Genètica Genes Mating Type Fungal Allosomes Genetic map DNA Transposable Elements autosomes genetic map PAR allosomes Gene Deletion Phylogeny Discoveries |
| Zdroj: | Molecular Biology and Evolution Molecular Biology and Evolution, 2015, 32 (4), pp.928-943. ⟨10.1093/molbev/msu396⟩ Recercat. Dipósit de la Recerca de Catalunya instname Molecular Biology and Evolution, Oxford University Press (OUP), 2015, 32 (4), pp.928-943. ⟨10.1093/molbev/msu396⟩ |
| ISSN: | 0737-4038 1537-1719 |
| Popis: | BGPI : équipe 5; International audience; Dimorphic mating-type chromosomes in fungi are excellent models for understanding the genomic consequences of recombination suppression. Their suppressed recombination and reduced effective population size are expected to limit the efficacy of natural selection, leading to genomic degeneration. Our aim was to identify the sequences of the mating-type chromosomes (a 1 and a 2) of the anther-smut fungi and to investigate degeneration in their nonrecombining regions. We used the haploid a 1 Microbotryum lychnidis-dioicae reference genome sequence. The a 1 and a 2 mating-type chromosomes were both isolated electrophoretically and sequenced. Integration with restriction-digest optical maps identified regions of recombination and nonrecombination in the mating-type chromosomes. Genome sequence data were also obtained for 12 other Microbotryum species. We found strong evidence of degeneration across the genus in the nonrecombining regions of the mating-type chromosomes, with significantly higher rates of nonsynonymous substitution (dN/dS) than in nonmating-type chromosomes or in recombining regions of the mating-type chromosomes. The non-recombining regions of the mating-type chromosomes also showed high transposable element content, weak gene expression, and gene losses. The levels of degeneration did not differ between the a 1 and a 2 mating-type chromosomes, consistent with the lack of homogametic/heterogametic asymmetry between them, and contrasting with X/Y or Z/W sex chromosomes. |
| Databáze: | OpenAIRE |
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