Construction of a map-based reference genome sequence for barley, Hordeum vulgare L
| Autor: | Beier, Sebastian, Himmelbach, Axel, Colmsee, Christian, Zhang, Xiao-Qi, Barrero, Roberto A., Zhang, Qisen, Li, Lin, Bayer, Micha, Bolser, Daniel, Taudien, Stefan, Groth, Marco, Felder, Marius, Hastie, Alex, Šimková, Hana, Staňková, Helena, Vrána, Jan, Chan, Saki, Muñoz-Amatriaín, María, Ounit, Rachid, Wanamaker, Steve, Schmutzer, Thomas, Aliyeva-Schnorr, Lala, Grasso, Stefano, Tanskanen, Jaakko, Sampath, Dharanya, Heavens, Darren, Cao, Sujie, Chapman, Brett, Dai, Fei, Han, Yong, Li, Hua, Li, Xuan, Lin, Chongyun, McCooke, John K., Tan, Cong, Wang, Songbo, Yin, Shuya, Zhou, Gaofeng, Poland, Jesse A., Bellgard, Matthew I., Houben, Andreas, Doležel, Jaroslav, Ayling, Sarah, Lonardi, Stefano, Langridge, Peter, Muehlbauer, Gary J., Kersey, Paul, Clark, Matthew D., Caccamo, Mario, Schulman, Alan H., Platzer, Matthias, Close, Timothy J., Hansson, Mats, Zhang, Guoping, Braumann, Ilka, Li, Chengdao, Waugh, Robbie, Scholz, Uwe, Stein, Nils, Mascher, Martin |
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| Přispěvatelé: | Institute of Biotechnology, Alan Schulman / Principal Investigator, Viikki Plant Science Centre (ViPS) |
| Rok vydání: | 2017 |
| Předmět: |
Statistics and Probability
Data Descriptor Plant genetics ASSEMBLIES Library and Information Sciences Education Genome assembly algorithms Genetics DNA sequencing Genome Human Genome 1184 Genetics developmental biology physiology food and beverages Chromosome Mapping Hordeum Plant FRAMEWORK LIBRARIES Computer Science Applications ALIGNMENT BIAS BACTERIAL ARTIFICIAL CHROMOSOMES Statistics Probability and Uncertainty PHYSICAL MAP Sequence Analysis Genome Plant Information Systems GENERATION |
| Zdroj: | Scientific data, vol 4, iss 1 Scientific data, 4:170044. Nature Publishing Group Scientific data, 4:170044 Scientific Data |
| ISSN: | 2052-4463 |
| Popis: | Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. `Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX). |
| Databáze: | OpenAIRE |
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