Genetic and Pathogenicity Diversity of Aphanomyces euteiches Populations From Pea-Growing Regions in France
| Autor: | Quillévéré-Hamard, Anne, Le Roy, Gwenola, Moussart, Anne, Baranger, Alain, Andrivon, Didier, Pilet-Nayel, Marie-Laure, Le May, Christophe |
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| Přispěvatelé: | Institut de Génétique, Environnement et Protection des Plantes (IGEPP), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), PISOM, Institut National de la Recherche Agronomique (INRA), Terres Inovia, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
legume crops
pouvoir pathogène Phytopathology and phytopharmacy food and beverages Plant Science Phytopathologie et phytopharmacie [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy aphanomyces euteiches diversité génétique genetic structure marqueur ssr pourriture des racines host specificity Aphanomyces root rot Pisum sativum Original Research |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2018, 9, pp.1673. ⟨10.3389/fpls.2018.01673⟩ Frontiers in Plant Science, 2018, 9, pp.1673. ⟨10.3389/fpls.2018.01673⟩ Frontiers in Plant Science (9), . (2018) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2018.01673⟩ |
| Popis: | International audience; Aphanomyces euteiches is an oomycete pathogen with a broad host-range on legumes that causes devastating root rot disease in many pea-growing countries and especially in France. Genetic resistance is a promising way to manage the disease since consistent QTL controlling partial resistance have been identified in near isogenic lines of pea. However, there are still no resistant pea varieties cultivated in France. This study aimed to evaluate the phenotypic and genetic diversity of A. euteiches populations from the major pea-growing regions in France. A collection of 205 isolates, from soil samples collected in infested pea fields located in five French regions, was established and genotyped using 20 SSR markers. Thirteen multilocus genotypes were found among the 205 isolates which displayed a low genotypic richness (ranged from 0 to 0.333). Two main clusters of isolates were identified using PCoA and STRUCTURE, including a predominant group comprising 88% of isolates and another group representing 12% of isolates mainly from the Bourgogne region. A subset of 34 isolates, representative of the fields sampled, was phenotyped for aggressiveness on a set of resistant and susceptible varieties of four legume hosts (pea, faba bean, vetch, alfalfa). Significant differences in disease severity were found among isolates and three groups of aggressiveness comprising 16, 17, and 2 isolates, respectively, were identified using HCA analysis. A higher diversity in pathogen aggressiveness was observed among isolates from Bourgogne, which included different legumes in its crop history. Little relationship was observed between genetic clusters and pathogenicity in the subset of 34 isolates, as expected using neutral markers. This study provides useful knowledge on the current state of low to moderate diversity among A. euteiches populations before resistant pea varieties are grown in France. New insights and hypotheses about the major factors shaping the diversity and evolution of A. euteiches are also discussed. |
| Databáze: | OpenAIRE |
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