Dissemination and Persistence of blaCTX-M-9 Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn402 Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-α, and IncFI Groups
| Autor: | Novais, Â, Cantón, R, Valverde, A, Machado, E, Galán, Jc, Peixe, L, Carattoli, A, Baquero, F, Coque, Tm. |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
chloramphenicol
kanamycin Enterobacter infection antibiotic resistance integron transposon DNA hybridization polymerase chain reaction retrospective study Variation (Genetics) plasmid incp 1alpha bacterial protein recombinase Integrons class 1 integron protein CR1 tertiary health care amikacin Tn antigen genetic variability antibiotic agent genetics hospital restriction fragment length polymorphism plasmid inci article Bacterial Enterobacteriaceae Infections Nucleic Acid Hybridization Salmonella enterica transposon 402 bla ctx m 9 gene Hospitals unclassified drug Klebsiella pneumoniae priority journal bacterium identification bacterium conjugation bacterial gene beta lactamase ciprofloxacin gentamicin mercury nalidixic acid streptomycin sulfonamide tetracycline tobramycin trimethoprim bacterial DNA beta lactamase antibiotic resistance antibiotic sensitivity bacterial strain bacterial transmission DNA fingerprinting DNA recombination DNA sequence Enterobacter cloacae Escherichia coli gene sequence Gram negative bacterium host range human integron 60 molecular evolution nonhuman nucleotide sequence plasmid plasmid incfi plasmid inch12 replicon Spain Enterobacteriaceae enzymology isolation and purification microbiology nucleic acid hybridization Spain beta-Lactam Resistance beta-Lactamases DNA Transposable Elements DNA Bacterial Humans Plasmids Retrospective Studies Sequence Analysis DNA Sequence Analysis beta-Lactam Resistance Mechanisms of Resistance Genetic Variation DNA |
| Popis: | This study analyzes the diversity of In60, a class 1 integron bearing CR1 and containing bla(CTX-M-9), and its association with Tn402, Tn21, and classical conjugative plasmids among 45 CTX-M-9-producing clinical strains (41 Escherichia coli strains, 2 Klebsiella pneumoniae strains, 1 Salmonella enterica strain, and 1 Enterobacter cloacae strain). Forty-five patients in a Spanish tertiary care hospital were studied (1996 to 2003). The diversity of In60 and association of In60 with Tn402 or mercury resistance transposons were investigated by overlapping PCR assays and/or hybridization. Plasmid characterization included comparison of restriction fragment length polymorphism patterns and determination of incompatibility group by PCR-based replicon typing, sequencing, and hybridization. CTX-M-9 plasmids belonged to IncHI2 (n = 26), IncP-1alpha (n = 10), IncFI (n = 4), and IncI (n = 1) groups. Genetic platforms containing bla(CTX-M-9) were classified in six types in relation to the In60 backbone and in eight subtypes in relation to Tn402 derivatives. They were associated with Tn21 sequences when located in IncP-1alpha or IncHI2 plasmids. Our study identified bla(CTX-M-9) in a high diversity of CR1-bearing class 1 integrons linked to different Tn402 derivatives, often to Tn21, highlighting the role of recombination events in the evolution of antibiotic resistance plasmids. The presence of bla(CTX-M-9) on broad-host-range IncP-1alpha plasmids might contribute to its dissemination to hosts that were not members of the family Enterobacteriaceae. |
| Databáze: | OpenAIRE |
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