Fast renal trapping of porcine Luteinizing Hormone (pLH) shown by 123I-scintigraphic imaging in rats explains its short circulatory half-life
| Autor: | Klett, Danièle, Bernard, Serge, Lecompte, François, Leroux, Hervé, Magallon, Thierry, Locatelli, Alain, Lepape, Alain, Combarnous, Yves |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Pathologie Infectieuse et Immunité, Institut National de la Recherche Agronomique (INRA), Protéases et vectorisation, Institut National de la Santé et de la Recherche Médicale (INSERM), The authors are grateful to the Région Centre Council for financial support., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Maylin, Françoise, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Male
MESH: Portal System Glycosylation Swine Kidney Chorionic Gonadotropin Iodine Radioisotopes Animals Comparative Study Female Half-Life Horses Injections Intravenous Liver Luteinizing Hormone N-Acetylneuraminic Acid Portal System Portasystemic Shunt Surgical Immunologie Biologie de la reproduction Portasystemic Shunt Surgical lcsh:Reproduction Tissue Distribution MESH: Animals [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Reproductive Biology MESH: Comparative Study MESH: Iodine Radioisotopes [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism MESH: Glycosylation Injections Intravenous Endocrinologie et métabolisme [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: N-Acetylneuraminic Acid MESH: Half-Life [SDV.IMM] Life Sciences [q-bio]/Immunology lcsh:QH471-489 Immunology lcsh:Gynecology and obstetrics Species Specificity MESH: Chorionic Gonadotropin MESH: Luteinizing Hormone MESH: Portasystemic Shunt Surgical Rats Wistar Radionuclide Imaging MESH: Horses [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology lcsh:RG1-991 Endocrinology and metabolism Research [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology MESH: Kidney MESH: Injections Intravenous MESH: Male Rats MESH: R Radiopharmaceuticals MESH: Female MESH: Liver |
| Zdroj: | Reproductive Biology and Endocrinology, Vol 1, Iss 1, p 64 (2003) Reprod Biol Endocrinol Reprod Biol Endocrinol, 2003, 1, pp.64. ⟨10.1186/1477-7827-1-64⟩ Reproductive Biology and Endocrinology (1), 64. (2003) Reproductive biology and endocrinology : RB&E |
| ISSN: | 1477-7827 |
| DOI: | 10.1186/1477-7827-1-64⟩ |
| Popis: | Background Sugar moieties of gonadotropins play no primary role in receptor binding but they strongly affect their circulatory half-life and consequently their in vivo biopotencies. In order to relate more precisely hepatic trapping of these glycoproteic hormones with their circulatory half-life, we undertook a comparative study of the distribution and elimination of porcine LH (pLH) and equine CG (eCG) which exhibit respectively a short and a long half-life. This was done first by following half-lives of pLH in piglets with hepatic portal circulation shunted or not. It was expected that such a shunt would enhance the short half-life of pLH. Subsequently, scintigraphic imaging of both 123I-pLH and 123I-eCG was performed in intact rats to compare their routes and rates of distribution and elimination. Methods Native pLH or eCG was injected to normal piglets and pLH was tested in liver-shunted anæsthetized piglet. Blood samples were recovered sequentially over one hour time and the hormone concentrations were determined by a specific ELISA method. Scintigraphic imaging of 123I-pLH and 123I-eCG was performed in rats using a OPTI-CGR gamma camera. Results In liver-shunted piglets, the half-life of pLH was found to be as short as in intact piglets (5 min). In the rat, the half-life of pLH was also found to be very short (3–6 min) and 123I-pLH was found to accumulate in high quantity in less than 10 min post injection at the level of kidneys but not in the liver. 123I-eCG didn't accumulate in any organ in the rats during the first hour, plasma concentrations of this gonadotropin being still elevated (80%) at this time. Conclusion In both the porcine and rat species, the liver is not responsible for the rapid elimination of pLH from the circulation compared to eCG. Our scintigraphic experiments suggest that the very short circulatory half-life of LH is due to rapid renal trapping. |
| Databáze: | OpenAIRE |
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