BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Autor:	Alpern, Daniel, Gardeux, Vincent, Russeil, Julie, Mangeat, Bastien, Meireles-Filho, Antonio C. A., Breysse, Romane, Hacker, David, Deplancke, Bart
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	lcsh:QH426-470 Sequence Analysis RNA Gene Expression Profiling genetic processes Method High-Throughput Nucleotide Sequencing qPCR lcsh:Genetics lcsh:Biology (General) natural sciences sense organs Gene expression RNA-seq Transcriptomics lcsh:QH301-705.5 Gene Library Barcoding
Zdroj:	Genome Biology, Vol 20, Iss 1, Pp 1-15 (2019) Genome Biology
Popis:	Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR. Electronic supplementary material The online version of this article (10.1186/s13059-019-1671-x) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::ddbc5cea6880178cd371a4d85f37274b http://link.springer.com/article/10.1186/s13059-019-1671-x Zobrazit plný text záznamu