Potentiation of glutamatergic agonist-induced inositol phosphate formation by basic fibroblast growth factor is related to developmental features in hippocampal cultures: neuronal survival and glial cell proliferation

Autor: Blanc, Emmanuelle, Jallageas, Monique, Recasens, Max, Guiramand, Janique
Přispěvatelé: Plasticité cérébrale (PC), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)
Rok vydání: 1999
Předmět:
MESH: Epidermal Growth Factor
MESH: Hippocampus
MESH: Neurons
Benzoates
Hippocampus
Receptors
Kainic Acid
Excitatory Amino Acid Agonists
MESH: Cytarabine
MESH: Animals
MESH: Receptors
Kainic Acid
Cells
Cultured
Cellular Senescence
Neurons
MESH: Antimetabolites
Antineoplastic
Cytarabine
MESH: Neuroprotective Agents
MESH: Excitatory Amino Acid Antagonists
MESH: Glutamic Acid
MESH: Glycine
Neuroprotective Agents
MESH: Cell Aging
MESH: Cell Survival
MESH: Receptors
AMPA
MESH: Cell Division
MESH: Neuroglia
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Fibroblast Growth Factor 2
MESH: Type C Phospholipases
Neuroglia
Cell Division
MESH: Cells
Cultured
Antimetabolites
Antineoplastic
MESH: Rats
Cell Survival
Inositol Phosphates
Glycine
Glutamic Acid
Fetus
MESH: Quinoxalines
Quinoxalines
Animals
Cycloleucine
Receptors
AMPA
MESH: Cycloleucine
MESH: Quisqualic Acid
MESH: Fibroblast Growth Factor 2
Epidermal Growth Factor
Quisqualic Acid
MESH: Fetus
MESH: Benzoates
MESH: Inositol Phosphates
Rats
Type C Phospholipases
MESH: Excitatory Amino Acid Agonists
Excitatory Amino Acid Antagonists
Zdroj: European Journal of Neuroscience
European Journal of Neuroscience, Wiley, 1999, 11 (10), pp.3377-86. ⟨10.1046/j.1460-9568.1999.00759.x⟩
ISSN: 0953-816X
1460-9568
DOI: 10.1046/j.1460-9568.1999.00759.x⟩
Popis: International audience; We investigated the modulation by growth factors of phospholipase C (PLC)-linked glutamate receptors during in vitro development of hippocampal cultures. In defined medium, glial cells represent between 3 and 14% of total cell number. When we added basic fibroblast growth factor (bFGF) 2 h after plating, we found: (i) a neuroprotection from naturally occurring death for up to 5 days; (ii) a proliferation of glial cells from day 3; and (iii) a potentiation of quisqualate (QA)-induced inositol phosphate (IP) formation from 1 to 10 days in vitro (DIV) and 1S, 3R-amino-cyclopentane-1,3-dicarboxylate (ACPD) response from 3 to 10 DIV. The antimitotic cytosine-beta,D-arabinofuranoside (AraC) blocked glial cell proliferation induced by bFGF, but not neuroprotection. Under these conditions, the early potentiation of the QA response (1-3 DIV) was not changed, while the ACPD and late QA response potentiations were prevented (5-10 DIV). Epidermal growth factor was not neuroprotective but it induced both glial cell proliferation and late QA or ACPD potentiation. Surprisingly, the early bFGF-potentiated QA-induced IP response was blocked by 6, 7-dinitro-quinoxaline-2,3-dione (DNQX), suggesting the participation of ionotropic (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)/kainate (KA) receptors. The delayed bFGF-potentiated ACPD-induced IP response is inhibited by (S)-alpha-methyl-4-carboxyphenylglycine (MCPG), indicating possible activation of glial metabotropic receptors. These results suggest that, in hippocampal cultures, bFGF modulates AMPA and metabotropic glutamate receptors linked to the IP cascade, possibly in relation to the regulation of neuronal survival and glial cell proliferation, respectively.
Databáze: OpenAIRE
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