Signal-dependent incorporation of MyoD–BAF60c into Brg1-based SWI/SNF chromatin-remodelling complex
| Autor: | Forcales, Sonia V, Albini, Sonia, Giordani, Lorenzo, Malecova, Barbora, Cignolo, Luca, Chernov, Andrei, Coutinho, Paula, Saccone, Valentina, Consalvi, Silvia, Williams, Roy, Wang, Kepeng, Wu, Zhenguo, Baranovskaya, Svetlana, Miller, Andrew, Dilworth, F Jeffrey, Puri, Pier Lorenzo |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chromosomal Proteins
Non-Histone MAP Kinase Signaling System cells genetic processes Muscle Proteins macromolecular substances Muscle Development p38 Mitogen-Activated Protein Kinases Article Cell Line Myoblasts Mice Two-Hybrid System Techniques Animals Humans Phosphorylation RNA Small Interfering MyoD Protein DNA Helicases Nuclear Proteins Fibroblasts Chromatin enzymes and coenzymes (carbohydrates) Phosphothreonine Gene Expression Regulation Multiprotein Complexes RNA Interference biological phenomena cell phenomena and immunity Protein Processing Post-Translational HeLa Cells Transcription Factors |
| Zdroj: | The EMBO Journal; Vol 31 |
| Popis: | Tissue-specific transcriptional activators initiate differentiation towards specialized cell types by inducing chromatin modifications permissive for transcription at target loci, through the recruitment of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodelling complex. However, the molecular mechanism that regulates SWI/SNF nuclear distribution in response to differentiation signals is unknown. We show that the muscle determination factor MyoD and the SWI/SNF subunit BAF60c interact on the regulatory elements of MyoD-target genes in myoblasts, prior to activation of transcription. BAF60c facilitates MyoD binding to target genes and marks the chromatin for signal-dependent recruitment of the SWI/SNF core to muscle genes. BAF60c phosphorylation on a conserved threonine by differentiation-activated p38α kinase is the signal that promotes incorporation of MyoD-BAF60c into a Brg1-based SWI/SNF complex, which remodels the chromatin and activates transcription of MyoD-target genes. Our data support an unprecedented two-step model by which pre-assembled BAF60c-MyoD complex directs recruitment of SWI/SNF to muscle loci in response to differentiation cues. |
| Databáze: | OpenAIRE |
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