Identification of spotted fever group rickettsiae using polymerase chain reaction and restriction-endonuclease length polymorphism analysis

Autor:	Drancourt M, Pj, Kelly, Regnery R, Didier Raoult
Rok vydání:	1992
Předmět:	Ticks Animals Centrifugation Rickettsia Polymerase Chain Reaction Vero Cells Polymorphism Restriction Fragment Length
Zdroj:	Europe PubMed Central
ISSN:	0001-723X
Popis:	In order to facilitate the isolation and identification of spotted fever group (SFG) rickettsiae from their tick vectors, we used the centrifugation shell vial technique or traditional isolation procedures and genotypic identification using the restriction fragment length polymorphism analysis of polymerase chain amplified fragments. The presence of Rickettsia conorii both in Rhipicephalus sanguineus ticks collected in southern France, and in Rhipicephalus simus and Haemaphysalis leachi from Zimbabwe was demonstrated. This procedure seems to be of particular interest for studying the epidemiology and ecology of SFG rickettsiae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::dc8633289b1c177eb7df33f5226a053a https://pubmed.ncbi.nlm.nih.gov/1350164 Zobrazit plný text záznamu