Thymosin beta-10 gene overexpression correlated with the highly malignant neoplastic phenotype of transformed thyroid cells in vivo and in vitro
| Autor: | D, Califano, C, Monaco, G, Santelli, A, Giuliano, M L, Veronese, M T, Berlingieri, V, de Franciscis, N, Berger, F, Trapasso, M, Santoro, G, Viglietto, A, Fusco |
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| Přispěvatelé: | D., Califano, C., Monaco, G., Santelli, A., Giuliano, M. L., Veronese, M. T., Berlingieri, V. d., Francisci, N., Berger, F., Trapasso, Santoro, Massimo, G., Viglietto, Fusco, Alfredo |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Cultured
Animals Carcinoma Carcinoma Papillary Thyroid Gland metabolism Thyroid Neoplasm Gene Expression Transfection Carcinoma Papillary Rats Inbred F344 Rats Inbred F344 Thymosin Thymosin genetics/metabolism Chromosome genetics/metabolism Transfection Tumor Cell genetics/metabolism Thyroid Gland genetics/metabolism Carcinoma Pair 2 Gene Expression Gene Library Humans Rats Rat Chromosomes Human Pair 2 Tumor Cells Cultured Animals Humans Thyroid Neoplasms Gene Library Human |
| Popis: | A subtractive thyroid cDNA library was constructed from two human thyroid carcinoma cell lines originating from an anaplastic carcinoma and a papillary thyroid carcinoma. The library was used to identify genes correlated with the progression to a highly malignant phenotype. The thymosin beta-10 gene was isolated and found to be expressed at much higher levels in the anaplastic cell line than in the papillary cells. The thymosin beta-10 gene was overexpressed in five carcinoma cell lines compared with normal thyroid tissue and normal thyroid primary culture cells. The highest expression occurred in the most malignant cell lines. Thymosin beta-10 gene expression was also increased in surgically removed human thyroid carcinomas and was highest in the anaplastic carcinomas. Thymosin beta-10 gene expression was correlated with the degree of the malignant phenotype also in rat thyroid cells transfected with cellular and viral oncogenes of different tumorigenicity. These results show that thymosin beta-10 overexpression is a general event of thyroid cell neoplastic transformation and suggest that the gene is involved in the progression of thyroid carcinogenesis. Finally, the thymosin beta-10 gene was located on chromosome 2q37 by fluorescence in situ hybridization analysis. |
| Databáze: | OpenAIRE |
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