ATF and Jun transcription factors, acting through an Ets/CRE promoter module, mediate lipopolysaccharide inducibility of the chemokine RANTES in monocytic Mono Mac 6 cells
| Autor: | Boehlk, S., Fessele, S., Mojaat, A., Miyamoto, N.G., Werner, T., Nelson, E.L., Schlöndorff, D., Nelson, P.J. |
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| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
Transcriptional Activation Proto-Oncogene Proteins c-jun Cells Messenger Immunology DNA Footprinting Response Elements Monocytes Substrate Specificity lipopolysaccharide RANTES Transcriptional regulation CRE element Monocyte Promoter Regions RANTES Genetic Genes Reporter Proto-Oncogene Proteins Tumor Cells Cultured Humans Deoxyribonuclease I transcriptional regulation RNA Messenger Promoter Regions Genetic Cyclic AMP Response Element-Binding Protein Reporter Chemokine CCL5 Cells Cultured Sequence Deletion Cultured Activating Transcription Factor 3 Base Sequence Proto-Oncogene Proteins c-ets lipopolysaccharide DNA Tumor Cells Transcription Factor AP-1 Genes monocyte Trans-Activators RNA CRE element Transcription Factors |
| Zdroj: | European journal of immunology, vol 30, iss 4 Boehlk, S; Fessele, S; Mojaat, A; Miyamoto, NG; Werner, T; Nelson, EL; et al.(2000). ATF and Jun transcription factors, acting through an Ets/CRE promoter module, mediate lipopolysaccharide inducibility of the chemokine RANTES in monocytic Mono Mac 6 cells.. European journal of immunology, 30(4), 1102-1112. doi: 10.1002/(sici)1521-4141(200004)30:4<1102::aid-immu1102>3.0.co;2-x. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8881w3qs Eur. J. Immunol. 30, 1102-12 (2000) |
| DOI: | 10.1002/(sici)1521-4141(200004)30:4<1102::aid-immu1102>3.0.co;2-x. |
| Popis: | The chemokine RANTES is produced by a variety of tissues, including cells of the monocyte/macrophage lineage. RANTES expression is rapidly and transiently up-regulated in primary monocytes and the monocytic cell line Mono Mac 6 in response to stimulation by the bacterial product lipopolysaccharide (LPS). Transient transfection of Mono Mac 6 cells with RANTES reporter-promoter deletion constructs, in conjunction with DNase I footprinting and heterologous reporter gene assays, allowed identification of an LPS-responsive region within the RANTES promoter. Electrophoretic mobility shift assays (EMSA), methylation interference and EMSA supershift experiments were used to characterize sequences and transcription factors responsible for this LPS inducibility. The region, termed RANTES site G [R(G)], contains consensus sites for Ets and CRE/AP-1-like elements. Site-directed mutagenesis of the Ets site resulted in a loss of only 15 % of promoter activity, while mutation of the CRE/AP-1 site led to a loss of 40 % of LPS-induced promoter activity. The Ets site constitutively binds the Ets family member PU.1. LPS stimulation leads to an induction of ATF-3 and JunD factor binding to the CRE/AP-1 site. Thus, LPS induction of RANTES transcription is mediated, in part, through the activation and selective binding of ATF and Jun nuclear factors to the R(G) promoter module. |
| Databáze: | OpenAIRE |
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